Análises in vitro de híbridos de borracha natural originada da Hevea brasilienses, na viabilidade e proliferação de osteoblastos murinos

Abstract

Natural rubber (NR) hybrid systems obtained from the latex of Hevea brasiliensis are the current target of many researchers due to their new and superior properties. To take advantage of these materials in the medical field, experiments to evaluate the cellular response are necessary. This manuscript presents a complete in vitro analysis of the biocompatible properties of two distinct hybrid systems developed from the association between NR and calcium phosphate (CaP) and silica particles (SiPs). We have produced NR films using two different organic solvents, chloroform (CHF) or toluene (TOL), and incorporated CaP or SiPs to investigate their effects on morphology, activity (alkaline phosphatase activity – ALP), and viability (MTT) of murine osteoblasts. The NR-Hybrids presented different heterogeneous and homogeneous distributions throughout the polymeric matrix dependent on the nature of the particles, which translates to affect surface wettability. Cell proliferation was increased at 48 and 72h with similar results observed after the addition of CaP. The cell morphologies are also affected by the hybridization process indicating the effects of both, solvent and particle nature. On the other hand, ALP activity was higher when NR was dissolved in CHF and enhanced after CaP or SiPs incorporation. The surface modification of NR films, dissolved in CHF or TOL, with the addition of CaP or SiPs, provided a different level of biocompatibility depending on the solvent, and charge. The effects on osteoblastic differentiation are correlated with preserved cell viability. Taken together, our data show that these novel NR hybrid systems may represent a very promising biological tool with desirable properties supporting bone augmentation.