Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/58414
Title in Portuguese: Preconditioning with L-Ala-Gln reduces the expression of inflammatory markers (TNF-α, NF-κB, IL-6 and HO-1) in an injury animal model of cerebrovascular ischemia in Meriones unguiculatus (gerbils)
Author: Oliveira, Leidelamar Rosário Alves de
Albuquerque, Andréa de Oliveira
Silva, Cícero Igor Simões Moura
Silva, Jussara Mathiele
Casadevall, Meyssa Quezado de Figueiredo Cavalcante
Azevedo, Orleancio Gomes Ripardo de
Albuquerque, Vilma Leite de Souza Pires
Vasconcelos, Paulo Roberto Leitão de
Keywords: Anti-Inflamatórios
Anti-Inflammatory Agents
Glutamina
Glutamine
Suplementos Nutricionais
Dietary Supplements
Imuno-Histoquímica
Immunohistochemistry
Issue Date: 2020
Publisher: Acta Cirurgica Brasileira
Citation: OLIVEIRA, Leidelamar Rosário Alves de et al. Preconditioning with L-Ala-Gln reduces the expression of inflammatory markers (TNF-α, NF-κB, IL-6 and HO-1) in an injury animal model of cerebrovascular ischemia in Meriones unguiculatus (gerbils). Acta Cir Bras., v. 35, n. 6, e202000601. Disponível em: https://www.scielo.br/pdf/acb/v35n6/1678-2674-acb-35-6-e202000601.pdf. Acesso em: 17/05/2020.
Abstract: Purpose: To evaluate the neuroprotective effect of L-alanyl-glutamine in a gerbil model of brain ischemia-reperfusion injury based on immunohistochemical quantification of pro-inflammatory and cell activation biomarkers (TNF-α, NF-κB, IL-6 and HO-1). Methods: Male gerbils weighing 100-180 g were pretreated with either 0.75 g/kg L-Ala-Gln (n=18) or 2.0 mL saline (n=18) administered i.v. 30 minutes before the bilateral ligation of the common carotid artery during 15 min and then the ligation was removed. Under anesthesia with urethane, brain tissue was harvested at 0 min (T0 ), 30 min (T30) and 60 min (T60) after reperfusion. The tissue was embedded in 10% formalin overnight and 4-μm sections were prepared for immunostaining with monoclonal antibodies. Immunostained cells were counted by optical microscopy. The statistical analysis used mean values based on 4 sections. Results: The pretreatment with L-Ala-Gln animal group 1 demonstrated significantly lower levels of TNF-α, NF-κB and IL-6. On the other hand, the levels of HO-1 were significantly higher, suggesting a protective role in model of brain ischemia-reperfusion injury. Conclusion: These findings suggest a protective effect of L-Ala-Gln by decreasing levels of TNF-alpha, IL-6 and NF-κB and Increasing levels of HO-1.
URI: http://www.repositorio.ufc.br/handle/riufc/58414
metadata.dc.type: Artigo de Periódico
ISSN: 0102-8650 (On-line)
Appears in Collections:PPGF - Artigos publicados em revistas científica

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