Frequência de Isolamentos dos Agentes Etiológicos da Mastite Bovina no Sudoeste Paranaense

Abstract

This study aimed to know the etiology of mastitis and the frequency of appearance of microorganisms in this in the Southwest of Paraná region. Was used the database of Agrifood Diagnostic Center Laboratory of Francisco Beltrão city. All reports of microbiological examination of milk, in the five years period were analyzed and tabulated, totaling 490 samples. It was observed that 2 (0.4%) samples showed no growth, and 97 (19.8%) presented contaminated. In the remaining, 391 samples of milk, we obtained a total of 539 pathogens isolates. Staphylococcus sp. was the most frequent (29.68%), followed by Enterobacteria (21.15%), Staphylococcus aureus (16.51%), Pseudomonas sp. (13.91%), Enterococcus sp. (4.64%), Bacillus sp. (3.71%), Streptococcus sp. (2.78%), Streptococcus dysgalactiae (2.41%), Streptococcus agalactiae (1.67%), Streptococcus uberis (1.3%), Nocardia sp. (0.93%), Prototheca sp. (0.56%), Candida albicans (0.37%), Corynebacterium sp. (0.19%) and Micrococcus sp. 0.19%). It was observed that during the first year of the study, infectious agents represented 60.42% of isolates, while in the fifth year was isolated greater amount of environmental microorganisms, totaling 70.71%. The bacterium Pseudomonas sp. was important for inversion of the scenario, increasing of 2.04% of the isolates in year one to 35.35% in year five. There were no significant differences between the pathogens to the seasons during the study period. This research showed that in Paraná Southwest region, mastitis cases are mainly caused by four types of etiologic agents: Staphylococcus sp, Enterobacteriaceae, S. aureus and Pseudomonas sp. Fifteen different species of microorganisms have been identified, including fungi and algae.