Enzyme adsorption and recycling during saccharification of pretreated cashewapple fiber

Abstract

The recycling of cellulase enzymes is one potential strategy for reducing the cost of the enzymatic hydrolysis step during the bioconversion of lignocellulosics to different products. In this context, the objective of this research was to investigate the recycle of cellulase during enzymatic hydrolysis of cashew apple fiber pretreated with acid followed by alkali. Different concentrations of hydrolyzed solids (HE-CAF) were evaluated in the recycle, and observed that using high quantity of HE-CAF was obtained higher glucose concentration, without enzymatic supplementation. These results indicate that occurred adsorption of free cellulases on pretreated cashew apple fiber and the supplementation of the hydrolysis reaction with fresh substrate was as a potential means of recovering the cellulases. The performances of results achieved with supplementation of enzyme were different, in a sense that it was obtained higher yield than without supplementation.