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metadata.dc.type: Artigo de Evento
Title in Portuguese: Octyl-nanoparticles: a new support to immobilize and stabilize lipases from Pseudomonas fluorescens
Title: Octyl-nanoparticles: a new support to immobilize and stabilize lipases from Pseudomonas fluorescens
Author: Rios, Nathalia Saraiva
Morais, Eva Gomes
Galvão, Wesley dos Santos
Santos, José Cleiton Sousa dos
Fechine, Pierre Basílio Almeida
Gonçalves, Luciana Rocha Barros
Issue Date: 2017
Keywords: Enzimas
Engenharia química
Citation: RIOS, Nathalia Saraiva.; MORAIS, Eva Gomes; GALVÃO, Wesley dos Santos; SANTOS, José Cleiton Sousa dos; FECHINE, Pierre Basílio Almeida; GONÇALVES, Luciana Rocha Barros. Octyl-nanoparticles: a new support to immobilize and stabilize lipases from Pseudomonas fluorescens. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, XXI; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, XII., 3 a 6 de set. 2017, Aracaju, Sergipe, Brasil. Anais [...] Aracaju, Sergipe, 2017.
Abstract in Portuguese: In this study, lipase from Pseudomonas fluorescens was immobilized on octyl-nanoparticles by hydrophobic interaction, producing the biocatalyst OCTYL-NANO-PSF. In order to stabilize the immobilized enzyme on OCTYL-NANO, an additional step was conducted, which is the reticulation with different concentrations of glutaraldehyde (GA), producing OCTYL-NANO-PSF-GA preparations. The concentrations of glutaraldehyde were 0.5, 1, 2.5 and 5 % v/v in sodium phosphate buffer, pH 7, 200 mM. The results show that the PSF could be immobilized on octyl-nanoparticles, events at short contact time between enzyme and support (Yield of immobilization about 75 %). The reticulation step was also shown successful, since the biocatalysts remain active (Activity retention above 55 %) and it was much more stable than OCTYL-NANO-PSF preparation. Best results of activity and stability was achieved when 5% was used (OCTYL-NANO-PSF-GA 5 %) for the reticulation. This biocatalyst was 65 fold more stable than adsorbed preparation (OCTYL-NANO-PSF).
ISSN: 2447 2816
Appears in Collections:DEQ - Trabalhos apresentados em eventos

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