Antitumour properties of the leaf essential oil of Xylopia frutescens Aubl. (Annonaceae)

Abstract

The aim of this study was to investigate the chemical composition and anticancer effect of the leaf essen- tial oil of Xylopia frutescens in experimental models. The chemical composition of the essential oil was analysed by GC/FID and GC/MS. In vitro cytotoxic activity of the essential oil was determined on cultured tumour cells. In vivo antitumour activity was assessed in Sarcoma 180-bearing mice. The major com- pounds identified were ( E )-caryophyllene (31.48%), bicyclogermacrene (15.13%), germacrene D (9.66%), d -cadinene (5.44%), viridiflorene (5.09%) and a -copaene (4.35%). In vitro study of the essential oil dis- played cytotoxicity on tumour cell lines and showed IC 50 values ranging from 24.6 to 40.0 l g/ml for the NCI-H358M and PC-3M cell lines, respectively. In the in vivo antitumour study, tumour growth inhi- bition rates were 31.0–37.5%. In summary, the essential oil was dominated by sesquiterpene constituents and has some interesting anticancer activity