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Title in Portuguese: Antigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis
Author: Medrano, Delia Jessica Astete
Cordeiro, Rossana de Aguiar
Patoilo, Kharla Rabelo Nobre
Praciano, Silviane Bandeira
Marques, Francisca Jakelyne de Farias
Martins, Liline Maria Soares
Eulalio, Kelsen Dantas
Deus Filho, Antônio de
Brilhante, Raimunda Samia Nogueira
Camargo, Zoilo Pires de
Rocha, Marcos Fábio Gadelha
Sidrim, José Júlio Costa
Fechine, Maria Auxiliadora Bezerra
Cavalvanti, Maria do Amparo Salmito
Keywords: Coccidioidomicose
Técnicas Imunoenzimáticas
Issue Date: Dec-2013
Publisher: Mycopathologia
Citation: MEDRANO, D. J. A. et al. Antigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis. Mycopathologia, São Paulo, v. 175, n.1-2, p. 25-32, dez. 2013.
Abstract: Serologic diagnosis has been presented as a safe alternative for coccidioidomycosis. However, commercial kits based on coccidioidal antibodies available in the USA are considered too expensive for laboratories outside that country. In this study, we describe the preparation of antigens for detection of human coccidioidal antibodies by the immunodiffusion test (ID) and enzyme immunoassay (EIA). Antigens were tested against serum samples from patients with coccidioidomycosis, histoplasmosis and paracoccidioidomycosis, as well as healthy individuals. The highest reactivity in the ID tests was seen in the F0-90 antigen. In the EIAs, the best results were obtained with the F60-90 antigen. None of the serum samples from healthy individuals were recognized by any of the antigen extracts tested by ID or EIA. In conclusion, the F0-90 and F60-90 antigens have the potential to be commercially employed in presumptive diagnosis of coccidioidomycosis by ID or EIA, respectively. The tests could improve serological diagnosis of coccidioidomycosis in South America.
metadata.dc.type: Artigo de Periódico
ISSN: 0301-486X Impressa
1573-0832 On line
Appears in Collections:DMC - Artigos publicados em revistas científicas

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