Potencial citotóxico da quinoxalina sintética, intitulada PACP 08A, na linhagem de carcinoma de células não pequenas de pulmão NCI-H460

Abstract

Cancer is responsible for a significant and growing number of deaths worldwide and is the second cause of death of the world's population. Synthetics products have taken more space in the research and in the pharmaceutical industry due to less environmental damage and the cost and production time reduce. This study evaluated the cytotoxic effects of the quinoxaline entitled PACP 08A on the viability of tumor cells, using in all experiments, doxorubicin as a positive control. Quinoxalines belong to the class of heterocyclic compounds from the group of benzodiazinas, although rarely found in nature they are easily synthesized. In particular they are employed as antitumor drugs, antibiotics, antifungal, anti-inflammatory, anticonvulsant and antiprotozoal. The IC50 values obtained from the MTT colorimetric assay varied from 0.77μM to 27.11μM in HL-60 and HEPG-2, respectively. Eight cell lines were used tumor cell lines of human origin and a murine fibroblast line (Mus musculus). The quinoxaline showed selectivity to eight tumor lines and expressed no cytotoxicity in normal peripheral blood mononuclear cells (PBMC) and also did not unfold membrane lysis. Due to the high epidemiological incidence and the lack of existing selective treatmentto to lung cancer, the following experiment followed with non-small cell lung cancer NCI-H460 lung. The exclusion of trypan blue showed that the IC50 and its twice, 16μM, exhibited antiproliferative effect, since it got fewer cells than the positive control. The other experiments, differential staining by LA / EB and staining by Panotic fast corroborate each other suggesting that the PACP 08A quinoxaline generates a necrotic process as a form of cell death in the above treatments mentioned.