Análise da frequência de polimorfismos genéticos de IL1RN, IL1β, IL6 e IL8 em lesão central de células gigantes

Abstract

Central Giant Cell Lesion is an intraosseous lesion, classified as aggressive and non-aggressive, of uncertain pathogenesis. There is no agreement on whether it is neoplastic or reactive, but the proliferative activity was demonstrated in stromal cells, suggesting the possibility of deregulation of the cell cycle in the pathogenesis. Objective: to analyse polymorphism in genes involved in inflammation in patients with the injury, among them, IL1 β -511C/T, IL1RN (VNTR), IL6 -174 G/C e IL8 -251 A/T. In order to do this, blood of 14 patients was collected. The presence of polymorphisms was determined by DNA extraction, by PCR and by PCR-RFLP. Of the cases studied, 10 were female and 4 were male, 10 were aggressive and 4 were non-aggressive, having a 1: 1 maxilla / mandible ratio. Among the 14 cases genotype, 10 were (L/L), 2 (L/2*) and 2 (2*/2*) for IL1RN. For IL1 β, 5 were (T/T), 7 (C/T) and 2 (C/C). For IL6, 10 (G/G), 3 (G/C), 1 (C/C) and for IL8, 3 (A/A), 8 (A/T), 3 (T/T). The importance of the polymorphic allele of IL6 in the LCCG protection condition (p = 0.00037) was verified. There was no statistical significance for any interleukin when comparing the aggressive and non-aggressive groups of the lesion, but in contrast, the IL6 had a significant difference when the aggressive and control groups were compared (p = 0.00024); and between the non-aggressive and control groups (p = 0.00065). Associations of IL6 with the IL1β -511C/T, IL1RN (VNTR) and IL8-251 A/T polymorphisms were also obtained for the genotype combinations in order to confirm the dominance of the polymorphic allele of IL6. It is concluded that cytokines may play a role in better understanding of the pathogenesis and clinical behavior, contributing to the understanding of genetic susceptibility and severity of the injury. However, further studies with larger sample relating other interleukins should be performed to clarify these correlations.