New flavone and other compounds from Tephrosia egregia: assessing the cytotoxic effect on human tumor cell lines

Abstract

The organic extracts from stems, roots and leaves of Tephrosia egregia Sandwith, Fabaceae, provided anew flavone, 5-hydroxy-8-(1 ,2 -epoxy-3 -hydroxy-3 -methylbutyl)-7-methoxyflavone (1), in additionto eleven known compounds: pongaflavone (2), praecansone B (3), 12a-hydroxyrotenone (4), praecan-sone A, 2 ,6 -dimethoxy-4 ,5 -(2 ,2 -dimethyl)-pyranochalcone, pongachalcone, maackiain, -sistosteroland its glucoside, p-cumaric acid and cinnamic acid. The structures of all compounds were establishedon the basis of spectroscopic methods, mainly 1D and 2D NMR and HRESIMS, involving comparison withliterature data. Cytotoxicity of compounds 1–4 was evaluated against AGP-01 (cancerous ascitic fluid),HCT-116 (colon adenocarcinoma), HL-60 (leukemia), PC-3 (prostate carcinoma), SF-295 (glioblastoma)and SKMEL 28 (melanoma) cell lines.