Síntese verde de nanopartículas de prata através de açúcares redutores e seu potencial antimicrobiano

Abstract

Silver nanoparticles (AgNPs) are promising materials in many areas of Science and technology. Traditional methods of synthesis generate undesirable and toxic residues. The green synthesis of AgNPs starting from reducing sugars emerges as a viable option of obtaining this material. At this present work, there were used the sugars arabinose, glucose, ribose and xylose to obtain AgNPs, as well as sodium citrate and sodium dodecyl sulfate as capping agents. The characterization of these materials were done trough the UV-Vis absorption – identifying the plasmon band – X-ray diffraction, dynamic light scattering, zeta potential, scanning electron microscopy, atomic force microscopy and infrared spectroscopy techniques. The solutions showed a good stability (mean zeta potential -46.4mV), particles size ranging from ±10nm to ±100nm and different grains morphology. Starting from synthesized AgNPs, materials – agar films and carbomer gel – were obtained to be characterized and applied. The application of AgNPs solutions and derivate materials was predominantly at verifying of microbiological activity against fungi of different species of Candida spp and Aspergillus spp, as well as pathogenic bacteria such Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. It was also investigated the effects of association between AgNPs and Itraconazole, a medicine to be used against fungal infections. All the nanoparticles applied showed antibiotic activity against the microorganisms, as well as the developed materials. The AgNPs showed also results that aim to the potentiation of Itraconazole, when combined with nanoparticles. The relation cost x benefits of the synthesis of AgNPs, its characteristics and applications turn the developed material on this research a potential adjunctive in the battle against multi-resistant microorganisms.