Please use this identifier to cite or link to this item:
Title in Portuguese: Immune cellular profile of bisphosphonate-related osteonecrosis of the jaw
Author: Silva, Paulo Goberlândio de Barros
Oliveira, C. C. de
Brizeno, Luiz André Cavalcante
Wong, Deysi Viviana Tenazoa
Lima Júnior, Roberto César Pereira
Alves, R. P. Gonçalves
Sousa, Fabrício Bitu
Mota, Mário Rogério Lima
Ribeiro, R. de Albuquerque
Alves, Ana Paula Negreiros Nunes
Keywords: Osteonecrose
Issue Date: Oct-2016
Publisher: Oral Diseases
Citation: SILVA, P. G. B. et al. Immune-cellular profile of bisphosphonate-related osteonecrosis of the jaw. Oral Diseases, Houndmills, v. 22, n. 7, p. 649-57, oct. 2016.
Abstract: OBJECTIVES: Characterize the cell profile and immunostaining of proinflammatory markers in an experimental model of bisphosphonate-related osteonecrosis of the jaw (BRONJ). MATERIALS AND METHODS: Male Wistar rats (n = 6-7) were treated chronically with saline solution or zoledronic acid (ZA) at 0.04, 0.20, and 1.00 mg kg(-1) (1.4 × 10(-7) , 6.9 × 10(-6) , and 3.4 × 10(-5) mol kg(-1) ), and subsequently, the first left inferior molar was extracted. Were performed counting of viable and empty osteocyte lacunae, viable and apoptotic osteoclasts, polymorphonuclear neutrophil, mast cells (toluidine blue), and the positive presence cells for CD68, tumor necrosis factor-alpha (TNF-α), IL (interleukin)-1β, inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-kB) and IL-18 binding protein (IL-18 bp). RESULTS: BRONJ was showed in ZA treated with 0.20 and 1.00 mg kg(-1) . There is a dose dependent increase in percentage of empty osteocyte lacunae (P < 0.001) and apoptotic osteoclasts (P < 0.001), counting of total osteoclasts (P = 0.003), polymorphonuclear neutrophil cells (P = 0.009), cytoplasmic-positive cells of CD68 (P < 0.001), TNF-α (P = 0.001), IL-1β (P = 0.001), iNOS (P < 0.001), NF-kB (P = 0.006), and nuclear-positive cells of NF-kB (P = 0.011). Consequently, there is no difference in mast cells (P = 0.957), and IL-18 bp immunostaining decreases dose dependently (P = 0.005). CONCLUSIONS: BRONJ is characterized by increases in immunostaining for proinflammatory markers and NF-kB and inversely associated with cells exhibiting IL-18 bp.
metadata.dc.type: Artigo de Periódico
ISSN: 1354-523X
Appears in Collections:DCOD - Artigos publicados em revistas científicas

Files in This Item:
File Description SizeFormat 
2016_art_pgbsilva.pdf1,58 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.