Carragenanas da alga marinha vermelha Solieria Filiformis: caracterização estrutural e avaliação da atividade cicatrizante

Abstract

In Red seaweeds (Rhodophyta) the sulfated polysaccharides are present mostly in the form of galactans sulfated subdividing themselves in carrageenan and agaranas. The study of these polysaccharides is justified by the fact that they are described in the literature as having various biological activities such as anticoagulant, antithrombotic, antioxidant, antiviral, anti-tumor, immunomodulatory, antinociceptive, anti-inflammatory, pro-inflammatory, gastroprotective, anxiolytic, among others. However, there are no reports of healing activity of sulfated polysaccharides from seaweed. In this study, we determined the structural characterization of carrageenan from red seaweed Solieria filiformis and evaluated the healing activity gels formulated with these carrageenan in model induced in mice wounds. The carrageenans were obtained by enzymatic extraction and fractionated by ion exchange chromatography on DEAE-cellulose matrix and characterized by infrared Fourier transform spectroscopy (FT-IR) and nuclear magnetic resonance (NMR), and the fractions (SF I and sf II) composed of a hybrid of k- and ι-carrageenan type. Both hybrid fractions showed no irritant potential dermal and healing activity model induced in mice wounds. However, the carrageenan consisting of kappa (30 %) and iota (70 %) at the concentration of 10 mg/mL, which was showed a better effect on healing. Thus suggesting a new biological applicability of said carrageenan in the healing process of cutaneous wounds.