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dc.contributor.authorLacerda, Rodrigo Rodrigues e-
dc.contributor.authorMoreira, Italo Cordeiro-
dc.contributor.authorNascimento, Jader Sabino Jacó do-
dc.contributor.authorLacerda, Ana Carenina Sampaio de-
dc.contributor.authorCabral, Natasha Lucena-
dc.contributor.authorLucetti, Daniel Luna-
dc.contributor.authorViana, Glauce Socorro de Barros-
dc.contributor.authorFelipe, Cícero Francisco Bezerra-
dc.contributor.authorPessoa, Hilzeth de Luna Freire-
dc.contributor.authorGadelha, Carlos Alberto de Almeida-
dc.contributor.authorSanti-Gadelha, Tatiane-
dc.identifier.citationLACERDA, R. R. e et al. Lectin isolated from Brazilian seeds of velvet bean (Mucuna pruriens (L) DC.) presents analgesic, anti-inflammatory and antihemolytic action. Journal of Medicinal Plants Research. v. 9, n. 8, p. 231-242, feb., 2015.pt_BR
dc.description.abstractLectins are proteins present in all living beings capable of interacting specifically and reversibly to carbohydrates or glycoconjugates form. They stand out in this family of lectins legumes, which are of special scientific interest because they have differen t biological functions. This study investigated the presence of a lectin in Brazilian seeds of Mucuna , proceeding with their isolation and evaluation of its antinociceptive effects, anti-inflammatory and antihemolytic activity. Saline extracts of Mucuna pruriens were investigated for the presence of hemagglutinating activity by serial dilution and applied to Sephacryl S-200HR columns and DEAE Sephacel HiPrepFF to purify their lectin. After assessing the purity and molecular weight by polyacrylamide agarose gel electrophoresis (PAGE), the hemolytic activity on human erythrocytes was determined, as well as its antinociceptive action by models of writhing induced by acetic acid and formalin, in addition to its anti-inflammatory activity by testing the paw edema induced by carrageenan. The results indicated that Brazilian Mucuna seeds had a lectin that was purified from seeds having molecular weight of 60.0 kDa and only able to agglutinate erythrocytes of rabbit. The lectin showed an antihemolytic effect on human erythrocytes by not causing hemolysis in these cells compared to the negative control. The administration of lectin in rodents intraperitoneally inhibited the writhing by 99% (2.5 mg/kg), induced by acetic acid, as well as in the method where the formalin nociceptive stimulus was reduced by 51% in stage one and 77% in stage two of the test, using 2.5 mg/kg lectin. The anti-inflammatory activity de monstrated a decrease in paw edema induced by carrageenan in 72% better result than indomethacin control where there was a reduction of only 48% of edema. The study supports the presence of a lectin in Brazilian seeds of M. pruriens with antinociceptive, anti-inflammatory and antihemolytic activities.pt_BR
dc.publisherJournal of Medicinal Plants Researchpt_BR
dc.subjectDolichos prurienspt_BR
dc.titleLectin isolated from Brazilian seeds of velvet bean ( Mucuna pruriens (L) DC.) presents analgesic, anti- inflammatory and antihemolytic actionpt_BR
dc.typeArtigo de Periódicopt_BR
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