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Type: Artigo de Periódico
Title: Brazilian red propolis : phytochemical screening, antioxidant activity and effect against cancer cells
Authors: Mendonça, Izabel Cristina Gomes de
Porto, Isabel Cristina Celerino de Moraes
Nascimento, Ticiano Gomes do
Souza, Naiana Soares de
Oliveira, José Marcos dos Santos
Arruda, Rodolfo Elleson dos Santos
Mousinho, Kristiana Cerqueira
Santos, Aldenir Feitosa dos
Basílio-Júnior, Irinaldo Diniz
Parolia, Abhishek
Barreto, Francisco Stefânio
Keywords: Compostos Fenólicos;Própole
Issue Date: Oct-2016
Publisher: BMC Complementary and Alternative Medicine
Citation: MENDONÇA, I. C. G. de et al. Brazilian red propolis : phytochemical screening, antioxidant activity and effect against cancer cells. BMC Complementary and Alternative Medicine, London, v. 15, p. 1-12, oct. 2015.
Abstract: Background: The implementation of new public healthcare models that stimulate the use of natural products from traditional medicine, as a so-called integrated medicine, refers to an approach that use best of both conventional medicine and traditional medicine. Propolis is a widely used natural product by different ancient cultures and known to exhibit biological activities beneficial for health. The large number of studies conducted with propolis had shown that its chemical composition differs as a function of the climate, plant diversity and bee species and plays an important role on its therapeutic properties. The aim of th is study was to analyse the phytochemical profile of the ethanolic extract of red propolis (EEP) and its fractionati on, antioxidant action of EEP and its fractions hexane, cloroform and ethyl acetate and cytotoxic activity of EEP on human tumour cell lines SF-295 (glioblastoma), OVCAR-8 (ovary) and HCT-116 (colon). Methods: EEP was obtained by maceration with absolute etha nol, then it was concentrated in rotaevaporator up to complete evaporation of the solvent. The crude extract was fractionated with hexane, ethyl acetate, chloroform and methanol and they were subjected to phytochemical screening and total phenolic compounds. Antioxidant activity of EEP and fractions was done by means of the 2,2-diphenyl-1-picryhydrazyl (DPPH) method. Biomarkers of red propolis were identified by LC-Orbitrap-FTMS. To assess cytotoxic activity of the extract, cells were exposed to EEP over 72 h. Cell viability was assessed by means of MTT assay. The percentage of cell growth inhibition (IC 50 )wasanalysedby means of non-linear regression, and the absorbance values of the various investigated concentrations were subjected to one-factor analysis of variance (ANOVA) followed by Tukey ’ sorTamhane ’ stests( α =0.05). Results: The results obtained using phytochemical screenin g and LC-Orbitrap-FTMS indicated the presence of phlobaphene tannins, catechins, chalcones, aurones, flavonones, flavonols, xanthones, pentacyclic triterpenoids and guttiferones in Brazilian red propolis. EEP and its he xane, chloroform and ethyl acetate fractions obtained by liquid-liquid partitioning exhibited sat isfactory antioxidant percentages. EEP (IC 50 <34.27 μ g/mL) exhibited high levels of cytotoxicity on all human tumour cell lines tested when compared to negative control. Conclusions: C-Orbitrap-FTMS was useful to establish the chemical profile of the red propolis. Brazilian red propolis has antioxidant properties and decreases substantially the percentage of cell survival of human tumour cells; thus, it has potential to serve as an anticancer drug.
ISSN: 1472-6882
Appears in Collections:PPGF - Artigos publicados em revistas científica

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