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Title in Portuguese: Easy storage strategies for sporothrix spp. strains
Author: Brilhante, Raimunda Sâmia Nogueira
Silva, Natalya Fechine
Lima, Rita Amanda Chaves de
Caetano, Érica Pacheco
Alencar, Lucas Pereira de
Castelo-Branco, Débora de Souza Collares Maia
Moreira, José Luciano Bezerra
Bandeira, Silviane Praciano
Camargo, Zoilo Pires de
Rodrigues, Anderson Messias
Bandeira, Tereza de Jesus Pinheiro Gomes
Monteiro, André Jalles
Cordeiro, Rossana de Aguiar
Sidrim, José Júlio Costa
Rocha, Marcos Fábio Gadelha
Keywords: Sporothrix
Issue Date: Apr-2015
Publisher: Biopreservation and Biobanking
Citation: BRILHANTE, R. S. N. et al. Easy storage strategies for sporothrix spp. strains. Biopreservation and Biobanking, v. 13, n. 2, p. 131-134, apr. 2015.
Abstract: The present study evaluated the maintenance of Sporothrix spp. (6 Sporothrix brasiliensis;6S. schenckii;5 S. mexicana, and 3 S. globosa) in saline at 4 C, and in 10% glycerol plus either 10% lactose or 10% sucrose, at -20 C and -80 C. Viability was assessed after 3, 6, and 9 months of storage, through the recovery of strains on potato dextrose agar and analysis of macro- and micromorphological features. Conidium quantification was performed before and after storage, at 3, 6 and 9 months. 100% viability was observed, regardless of storage conditions or time period. Storage at 4 C and at -20 C did not alter the number of conidia, but lower conidium counts were observed at -80 C. This study shows that the combination of glycerol with lactose or sucrose is effective to maintain Sporothrix spp. at freezing temperatures.
metadata.dc.type: Artigo de Periódico
ISSN: 1947-5535
Appears in Collections:DPML - Artigos publicados em revista científica

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