Please use this identifier to cite or link to this item:
Type: Artigo de Periódico
Title: In vitro inhibitory activity of terpenic derivatives against clinical and environmental strains of the Sporothrix schenkii complex
Authors: Brilhante, Raimunda Sâmia Nogueira
Marques, Natalya Fechine Silva
Castelo-Branco, Débora de Souza Collares Maia
Lima, Rita Amanda Chaves de
Malaquias, Angela Donato Maia
Caetano, Erica Pacheco
Barbosa, Giovanna Riello
Camargo, Zoilo Pires de
Rodrigues, Anderson Messias
Monteiro, André Jalles
Bandeira, Tereza de Jesus Pinheiro Gomes
Cordeiro, Rossana de Aguiar
Sidrim, José Júlio Costa
Moreira, José Luciano Bezerra
Rocha, Marcos Fábio Gadelha
Silva, Natalya Fechine
Keywords: Esporotricose;Iodeto de Potássio
Issue Date: 2015
Publisher: Medical Mycology
Citation: BRILHANTE, R. S. N. et al. In vitro inhibitory activity of terpenic derivatives against clinical and environmental strains of the Sporothrix schenkii complex. Medical Mycology, Oxford, v. 53, p. 93-98, 2015.
Abstract: Sporotrichosis is a subacute or chronic subcutaneous infection, caused by the fungus Sporothrix schenkii complex, occurring in human and animal tissues. Potassium iodide and itraconazole have been used as effective therapy for first-choice treatment, while am- photericin B may be indicated for disseminated infection. However, the adverse effects of potassium iodide and amphotericin B or the long duration of therapy with itraconazole often weigh against their use, leading to the search for alternatives for the treatment of severe infections. Terpinen-4-ol and farnesol are components of essential oils present in many plant species and have been described to have antifungal activity against mi- croorganisms. In this study, 40 strains of Sporothrix spp. were tested for the suscep- tibility to terpinen-4-ol and farnesol. Changes in cytoplasmic membrane permeability were also investigated. Terpenes inhibited all Sporothrix strains with MIC values ranging from 87.9 to 1,429.8 μ g/ml for terpinen-4-ol and from 0.003 to 0.222 μ g/ml for farnesol. The MFC values ranged from 177.8 to 5,722.6 μ g/ml and from 0.027 to 0.88 μ g/ml, re- spectively, for terpinen-4-ol and farnesol. Farnesol was the most active compound for the Sporothrix strains. Significant loss of 260 and 280 nm-absorbing material did not occur after treatment with concentrations equivalent to the MIC and sub-MIC of the tested ter- penes, when compared to corresponding untreated samples. The failure of terpenes to lyse Sporothrix cells suggests that their primary mechanism of action is not by causing irreversible cell membrane damage. Thus, new studies are needed to better understand the mechanisms involved in the antifungal activity.
ISSN: 1369-3786
Appears in Collections:DPML - Artigos publicados em revista científica

Files in This Item:
File Description SizeFormat 
2015_art_rsnbrilhante.pdf741,26 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.