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Title in Portuguese: Cross-resistance to fluconazole induced by exposure to the agricultural azole tetraconazole : an environmental resistance school?
Author: Rocha, Marcos Fabio Gadelha
Alencar, Lucas Pereira de
Araújo Neto, Manoel Paiva de
Melo, Luciana Magalhães
Bandeira, Silviane Praciano
Ponte, Y. B.
Sales, Jamille Alencar
Guedes, G. M. M.
Castelo-Branco, Débora de Souza Collaris Maia
Bandeira, Tereza de Jesus Pinheiro Gomes
Cordeiro, Rosana de Aguiar
Pereira-Neto, Waldemiro de Aquino
Brandine, G. S.
Moreira, José Luciano Bezerra
Sidrim, José Júlio Costa
Brilhante, Raimunda Sâmia Nogueira
Keywords: Fluconazol
Issue Date: Feb-2016
Publisher: Mycoses
Citation: ROCHA, M. F. G. et al. Cross-resistance to fluconazole induced by exposure to the agricultural azole tetraconazole : an environmental resistance school?. Mycoses, Berlin, p. 1-10, 2016.
Abstract: This study aimed to investigate the influence of tetraconazole and malathion, both used in agricultural activities, on resistance to fluconazole, itraconazole and voriconazole in Candida parapsilosis ATCC 22019. The susceptibility to tetraconazole, malathion, fluconazole, itraconazole and voriconazole, through broth microdilution. Then, 12 independent replicates, were separated and exposed to four treatment groups, each one containing three replicates: G1: tetraconazole; G2: malathion; G3: fluconazole (positive control); G4: negative control. Replicates from G1, G2 and G3, were exposed to weekly increasing concentrations of tetraconazole, malathion and fluconazole, respectively, ranging from MIC/2 to 32 9 MIC, throughout 7 weeks. The exposure to tetraconazole, but not malathion, decreased susceptibility to clinical azoles, especially fluconazole. The tetraconazole-induced fluconazole resistance is partially mediated by the increased activity of ATP-dependent efflux pumps, considering the increase in antifungal susceptibility after the addition of the efflux pump inhibitor, promethazine, and the increase in rhodamine 6G efflux and CDR gene expression in the G1 replicates. Moreover, MDR expression was only detected in G1 and G3 replicates, suggesting that MDR pumps are also involved in tetraconazoleinduced fluconazole resistance. It is noteworthy that tetraconazole and fluconazoletreated replicates behaved similarly, therefore, resistance to azoles of clinical use may be a consequence of using azoles in farming activities.
metadata.dc.type: Artigo de Periódico
ISSN: 1439-0507 On line
Appears in Collections:DPML - Artigos publicados em revista científica

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