Avaliação do Potencial Lipídico, Protéico e Antioxidante de Resíduos de Indústrias de Processamento de Frutas e Emprego de CO2 Supercrítico no Estudo de Metabólitos Secundários

Abstract

This work presents the results obtained in the evaluation of the purport of protein, lipid and antioxidant potential of the acerola seeds (Malphigia glabra), graviola (Annona muricata), passion fruit (Passiflora edulis), oiti (Moquilea tomentosa), mango (Mangifera indica), sapoti (Achras sapota), guava (Psidium guajava), plum (Prunus domestica), papaya (Carica papaya), ciriguela (Spondias purpurea), strawberry (Fragaria vesca), murici (Byrsonyma crassifolia), pineapple (Ananas comosus) and tamarind (Tamarindus indica) and study of secondary metabolites of the passion fruit seed using supercritical CO2 as process of selective extraction. The tenor of protein was evaluated by the bradford method, it is treated of a spectrofotometric method that consists of evaluating the variation of the absorbance of the comassie brilliant blue color. The antioxidant potential was certain by the techniques of DHBA (based on the inhibition of the formation of the acid di-hidróxibenzóico) and DPPH (it consists of the capture of the free radical DPPH). The tenor of lipid was evaluated by the analysis in GC/MS and GC/FID of the fatty acids obtained by extraction in hexane and in supercritical CO2. The samples presented considerable tenor of protein and lipid, as well as, antioxidant activity in both methods. Was studied the secondary metabolites of the passion fruit seed that they were extracted selectivity for CO2 in supercritical state, changing conditions of temperature, pressure and co-solvent content to select classes of compositions in the extraction.