Análise do perfil de proteínas salivares de crianças com sobrepeso e obesidade do Instituto da Primeira Infância : IPREDE no estado Ceará

Abstract

Obesity is a recurring theme in today's scientific literature. This is due to the exponential increase in its prevalence in all layers of society. The popularity of this theme also made matters associat ed with it emerged and gained greater notability in healthcare publications. The use of saliva as a diagnostic method has advanced considerably in recent years. Imbalances in the quantity and quality of saliva can generate both oral diseases as being indic ative of some important systemic change. This research studies the salivary protein profile and total human saliva in patients with overweight and obesity. The sample consisted of sixty patients with obesity and overweight (experimental group) and sixty pa tients with normal weight (control group) and was rated the salivary flow, the diet diary and the protein profile. Unstimulated saliva was collected and stored at - 80 ° C. Subsequently the enzyme inhibitor was added and the samples were centrifuged at 15, 000 rpm for 15 minutes at 4 ° C and the separated supernatant to perform protein dosing. The salivary concentration of total proteins was determined by the bicinchoninic acid method using a curve of bovine serum albumin (BSA). When analyzing the unstimulat ed salivary flow was observed that the study group had a mean less than the control group, and this difference was statistically significant (p = 0.006). The control group had an average total protein concentration greater than the experimental group was s tatistically significant (p = 0.002). The results of this study suggest different patterns in salivary composition between these two groups.