http://repositorio.ufc.br/handle/riufc/394 2026-06-13T00:16:44Z http://repositorio.ufc.br/handle/riufc/86530 Título: Investigação de testes de sarcopenia como preditores de quedas na doença de Parkinson leve a moderada: estudo de coorte prospectivo Autor(es): Gomes, Vlademir Carneiro Abstract: Parkinson's Disease (PD) is the second most prevalent neurodegenerative disease in Brazil, affecting 1% of the elderly population aged over 65 years and 4 to 5% of those aged over 85 years. It is a chronic and progressive central nervous system disorder associated with a decreased quality of life and functional impairment. The prevalence of falls in elderly individuals with PD is higher than in the general population, with an annual risk of falls ranging from 45% to 68%. It is known that there are differences between single fallers and recurrent fallers, but there are limited studies addressing this context. Sarcopenia is a complex and multifactorial condition characterized by a progressive and generalized reduction in the quantity and quality of skeletal muscle tissue. Currently considered a global public health issue, sarcopenia is associated with an increased risk of falls, fractures, functional impairment, and mortality in the elderly. The objective of this study was to investigate predictive factors for recurrent and non-recurrent falls based on the diagnostic criteria of sarcopenia in patients with mild to moderate Parkinson's Disease. This was a cohort study conducted at the Hospital Universitário Walter Cantidio in Fortaleza, Ceará, with patients from the Movement Disorders outpatient clinic of the Neurology department from March 2021 to March 2023. To be eligible, patients needed to have a confirmed diagnosis of PD, a Hoehn and Yahr severity stage between 1 and 3, and the ability to stand and walk independently. Individuals with severe medical problems or uncontrolled chronic diseases were excluded. Demographic and clinical data, anthropometric measurements, cognitive assessment using the Mini-Mental State Examination, evaluation of depressive symptoms using the Geriatric Depression Scale, assessment of parkinsonian symptoms using the Unified Parkinson's Disease Rating Scale, fall history, and sarcopenia assessment based on the Revised European Consensus on Sarcopenia recommendations were collected during the patients' regular appointments. Each patient received a questionnaire to fill out in case of falls during the 12-month period. Monthly phone calls were made to check for fall occurrences. The study sample consisted of 103 patients, of whom 38 (37%) were women and 65 (63%) were men, with an average age of 66 ± 10.5 years. Higher SARC-F scores and a longer disease duration were independent predictors of recurrent and non-recurrent falls over a 12-month period. Tipo: Dissertação 2023-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/86129 Título: Efeitos da liraglutida, um agonista do receptor do peptídeo-1 semelhante ao glucagon (GLP-1), sobre parâmetros metabólicos e saciedade de pacientes com lipodistrofia generalizada congênita Autor(es): Araújo, Jéssica Silveira Abstract: Lipodystrophies comprise rare, often underdiagnosed conditions characterized by partial or complete loss of subcutaneous adipose tissue, resulting in ectopic fat deposition in organs such as the pancreas, skeletal muscle, and liver, which may lead to severe metabolic complications. The spectrum of these complications is directly related to the extent of body fat loss, with generalized forms exhibiting more pronounced metabolic impairment and hyperphagia driven by markedly low leptin levels. The literature on the effects of glucagon-like peptide-1 receptor agonists in patients with lipodystrophies remains limited. Therefore, this study represents the first to evaluate the effects of liraglutide on metabolic parameters and satiety in patients with congenital generalized lipodystrophy (CGL). This open-label interventional study included patients with CGL followed by the Brazilian Group for the Study of Hereditary and Acquired Lipodystrophies (BRAZLIPO), who received liraglutide for 12 weeks. Metabolic outcomes were assessed at baseline, after treatment (week 12), and following an additional 12-week off- treatment period (week 24), with complementary evaluation of body composition, satiety, and hepatic imaging. Changes over time were analyzed using the Friedman test, with a significance level set at 5%. Eight patients were included (aged 24–45 years, six females); gastrointestinal adverse events led to treatment discontinuation in three patients. After 12 weeks of liraglutide therapy, a median reduction in HbA1c (−2.5%; range 1.1 to 3.1%; p=0.012) and in total daily insulin dose (−20%; range 0 to 100%; p=0.042) was observed, along with a non-significant reduction in triglycerides (−127 mg/dL; range 64 to 1171 mg/dL; p=0.350). Following treatment discontinuation, all patients exhibited increases in HbA1c and body weight of 2.2% (range 0.8 to 3.5%; p=0.026) and 3.6% (range 3.5 to 4.7%; p=0.001), respectively. Triglyceride levels increased in 80% of patients (median 421.5 mg/dL; range 76 to 1649; p=0.334). Insulin requirements also increased overall, with one patient resuming insulin at 0.4 U/kg/day and the others showing a median dose increase of 22% (range 12 to 50%; p=0.024). This study provides the first evidence that liraglutide may confer metabolic benefits in patients with CGL. Larger and longer-term studies are warranted to better characterize its effects on satiety, body composition, and liver disease in this population. Tipo: Dissertação 2026-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/85823 Título: Micropartículas de quitosana e óleo essencial de cúrcuma no controle in vitro de cryptococcus spp.: atividade antifúngica em células planctônicas, biofilmes e interação com antifúngicos Autor(es): Silva, Maria Isabel de Souza Abstract: Species of the Cryptococcus neoformans/Cryptococcus gattii complex are encapsulated yeasts responsible for cryptococcosis, an opportunistic mycosis that affects humans and animals, particularly immunocompromised individuals. Planktonic and biofilm susceptibilities, the interaction with classical antifungal agents already used in the treatment of cryptococcosis, and the cytotoxicity profile of chitosan microparticles associated with turmeric essential oil against species of the Cryptococcus neoformans/Cryptococcus gattii complex were evaluated. Planktonic susceptibility and mature biofilm susceptibility assays were performed following standardized protocols, using crystal violet staining for biomass quantification and the MTT assay to assess metabolic activity. In addition, for chitosan microparticles loaded with turmeric essential oil, the checkerboard method was employed to evaluate interactions with classical antifungal agents, and cytotoxicity was assessed through cell viability assays in cell cultures. Statistical analyses were performed considering p < 0.05. After exposure to different concentrations, the minimum inhibitory concentration (MIC) ranges for chitosan microparticles containing turmeric essential oil were 4–256 μg/mL for 50% inhibition and 256–1024 μg/mL for 100% inhibition. For mature biofilms, the main tested compound was able to inhibit up to 33% of biomass at the highest concentration (256 μg/mL) and to reduce metabolic activity by up to 42% at the same concentration. The test compound showed an indifferent interaction when combined with amphotericin B and fluconazole, with no evidence of synergism or antagonism. Cytotoxicity was observed at a concentration of 400 μg/mL, reducing cell viability by nearly 75%. Finally, chitosan microparticles loaded with turmeric essential oil demonstrated antifungal activity against planktonic cells and mature biofilms of the Cryptococcus neoformans/Cryptococcus gattii species complex in in vitro assays. Tipo: Dissertação 2026-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/85405 Título: Células dendríticas foliculares: avaliação imunofenotípica no linfoma de Hodgkin clássico subtipo esclerose nodular Autor(es): Almeida, Juliêta Maria Mendes Frota de Abstract: The follicular dendritic cells are the most important antigen presenting cells and have an important role in B cell immune response. Classic Nodular Sclerosis Hodgkin's Lymphoma (CNSHL) is a lymphoid neoplasm, more specifically from germinal center (GC) B cells, presenting with aggregates of follicular dendritic cells (FDC), Hodgkin/Reed Sternberg cells and variants and B cells forming complexes related to the GC, suggesting an association between nodular sclerosis and germinal center formation. CNSHL represents 70% of classic Hodgkin's lymphomas and has a favorable prognosis. Histologically it is characterized by a nodular pattern, collagen bands and lacunar cells. The goal of this study was the evaluation of the follicular dendritic cell by immunophenotyping with pS100 and fascin, in lymph node or mediastinal mass biopsies from patients with previously diagnosed classic nodular sclerosis Hodgkin's Lymphoma, trying to identify criteria as prognostic factors. 159 charts were reviewed and 38 patients were selected for the study. From the selected group 55.2% were males with a male to female ratio of 1.23:1 and a median age of 29.3 years. 52.6% were in clinical stage 1-11 and 68.4% had systemic symptoms (B). Multiple or isolated peripheral adenomegaly was present in all cases. The first lymph node site was cervical in 58% of the cases. 38 biopsy specimens were analysed and 57.9% were of nodular sclerosis subtype II. Immunohistochemistry showed 100% positivity for CD30 and 68.4% for CD15. The follicular dendritic cells were identified by pS100 and fascin antibodies through the Peroxidase-antiperoxidase Streptavidin avidin-biotin indirect immunoenzimatic technique, which was performed in pre-treated slides with the biopsy specimens included in paraffin blocks. The marking with pS100 didn't show good visualization of the network of follicular dendritic cells, and was useful as a generic marker. Fascin was the gold-standard for follicular dendritic cells, showing its positivity by the brown color. The CDF1 pattern was present in 7.9%, CDF2 in 47.4% and CDF3 in 44.7%. There was no relation between the presence of the follicular dendritic cell and sex, age, clinical stage, laboratorial parameters, or response to treatment, but a tendency for the association (p=0.056) between the subtypes of classical nodular sclerosis Hodgkin's lymphoma was demonstrated. The patients with follicular dendritic cells present in their biopsies have been watched for a longer period of time of about 32.9 months. This enabled a significant statistical association (p=0.001) between the presence of follicular dendritic cells and length of follow-up Tipo: Dissertação 2004-01-01T00:00:00Z