http://repositorio.ufc.br/handle/riufc/389 2026-06-10T00:45:16Z http://repositorio.ufc.br/handle/riufc/86620 Título: Correlação da incontinência fecal com parâmetros clínicos, funcionais e anatômicos de pacientes do sexo feminino submetidas à secção da musculatura esfincteriana lisa pós fistulotomia anal e esfincterotomia anal Autor(es): Mont’Alverne, Ricardo Everton Dias Abstract: Introduction: Anal fistula and anal fissure are benign diseases affecting the anal canal; however, surgical treatment may be required, often involving division of the internal sphincter muscle. As a consequence, patients may experience sequelae that significantly impact their quality of life, such as fecal incontinence, with female patients being particularly susceptible. Methodology: Sixty-three female patients who underwent internal sphincter muscle division through anal fistulotomy (30 patients) or anal sphincterotomy (33 patients) were evaluated. Clinical assessment was performed using a fecal incontinence score, functional assessment by anorectal manometry, and anatomical assessment by 3D endoanal ultrasound. Results: Overall, 46% (29/63) of patients reported symptoms of fecal incontinence after internal anal sphincter division, with 18 (60%) in group I and 16 (48%) in group II. However, the severity of symptoms was similar between groups, with mean scores of 1.6 (0–8) in group I and 2 (0–7) in group II. Resting pressure decreased in both groups and was similar between them postoperatively. The longitudinal length and percentage of the internal sphincter muscle divided were greater in group I. The longitudinal length of the anterior external anal sphincter and the distance between the external anal sphincter and puborectalis muscle were similar in both groups. However, the gap length—the distance between the proximal edge of the external anal sphincter and the distal edge of the puborectalis—was significantly greater in group II. Conclusion: Sphincter division surgery, regardless of the operative technique, is associated with a high rate of fecal incontinence, although the symptom severity remains low. The length and percentage of smooth muscle divided were smaller in patients who underwent anal sphincterotomy. Nevertheless, the rate of fecal incontinence was similar between the two groups. This finding may be explained by the longer gap observed in this group, leading to greater asymmetry of the anal canal. Tipo: Dissertação 2025-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/86591 Título: Uma narrativa autobiográfica da experiência como mediadora da intervenção relacional Autor(es): Aguiar, Otaciana Araújo de Abstract: This paper presents an autobiographical narrative of a Relational Intervention mediator, aiming to report her experience and the effects of this intervention on her personal and professional growth, as well as on the quality of relationships between caregivers and children. Our objective is to gather elements that promote reflection on narrative as a source and methodological tool for research, contributing to the theoretical and procedural aspects of the use of the researcher's own autobiographical narrative. The methodological approach used is based on the qualitative and interpretative approach, from the perspective of narrative phenomenology, in which the author is both protagonist and character. As a theoretical foundation, we used the concepts of Bertaux (2010). By examining the dynamics and challenges faced, this study offers a comprehensive perspective on the effectiveness of relational intervention, contributing to a deeper and applied understanding in the field of developmental psychology and psychotherapy. Justification: The quality of relationships between caregivers and children is a crucial component for the healthy emotional and psychological development of children. Relational intervention, as developed by Chantal Cyr, based on attachment theory, emerges as an innovative and effective approach to improving these interactions. The need to explore the effects of this approach not only on the caregiver/child relationship, but also on the development of the mediators themselves, is fundamental to expanding knowledge in this field. This study is justified by its ability to provide detailed and practical insights into relational intervention practices, enriching the existing literature with a personal narrative that contextualizes the challenges and benefits of this practice. Through this investigation, we seek to inspire and inform researchers and professionals in the field, promoting greater adoption and refinement of relational intervention techniques. Tipo: Dissertação 2024-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/86557 Título: Exposição pré-natal ao SARS-CoV-2, ativação imune materno-fetal e desfechos do neurodesenvolvimento infantil Autor(es): Kehdi, Renata Castro Abstract: This thesis investigates the relationship between maternal SARS-CoV-2 infection and the expression of pro-inflammatory markers in children up to 18 months of age. The research bases itself on the theory that the activation of the maternal immune system during pregnancy (MIA), due to exposure to pathogens such as SARS-CoV-2, can lead to the release of pro-inflammatory cytokines that remodel fetal brain connections, increasing the risk of neurodevelopmental disorders. In terms of methodology, the investigation uses the PLACOVGEN-BR cohort from the Neuropsychopharmacology Laboratory (LABNEURO/NPDM/UFC) in collaboration with the Assis Chateaubriand School Maternity (MEAC/UFC) and the Nucleus for Early Treatment and Stimulation (NUTEP), collecting data from pregnant women and their children between 2021 and 2023, involving the measurement of cytokines and chemokines in biospecimens via multiplex assay and the clinical evaluation of offspring neurodevelopment using gold-standard scales, such as the Bayley scale, to analyze cognitive, motor, and communication domains. The findings showed that prenatal exposure to SARS-CoV-2 was associated with a high frequency of neurodevelopmental delays at 24 months, particularly in the communication and motor domains. An increasing frequency of delays was observed between 6 and 24 months, especially in communication. Additionally, elevated levels of cytokines and chemokines in umbilical cord blood were associated with the observed outcomes, suggesting that perinatal inflammatory mediators, such as IFN-γ, TNF-α, IL-6, IL-8, IL-17, IL-1β, and CXCL10, may serve as early risk biomarkers for neurodevelopmental alterations in children exposed in utero to SARS-CoV-2. Taken together, these findings indicate that prenatal exposure to SARS-CoV-2 may adversely affect infant neurodevelopment, possibly through mechanisms involving maternal-fetal immune activation. These results reinforce the importance of longitudinal follow-up of children exposed during pregnancy and suggest the potential of perinatal inflammatory biomarkers for early risk identification. Tipo: Tese 2026-01-01T00:00:00Z http://repositorio.ufc.br/handle/riufc/86546 Título: O receptor P2X7 microglial orquestra neuroinflamação, disfunção autofágica e sinucleinopatia em ratos parkinsonianos Autor(es): Nascimento, Tyciane de Souza Abstract: Parkinson’s disease (PD) is a multisystem neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons in the substantia nigra and the presence of protein inclusions known as Lewy bodies, which are primarily composed of aggregated α-synuclein. Under physiological conditions, these proteins are targeted for autophagic degradation. Hyperactivity of purinergic P2X7 receptors has been extensively implicated in the pathophysiology of PD, with growing evidence supporting their role in autophagic dysfunction and protein aggregation. Thus, the present study aimed to investigate the neuroprotective effect of the P2X7 receptor antagonist Brilliant Blue G (BBG), administered during a prodromal phase of PD, focusing on its impact on α-synuclein accumulation and dysfunction of autophagic pathways in an experimental model of rotenone-induced parkinsonism. For this purpose, male Wistar rats were divided into four groups: control, control treated, parkinsonian (rotenone 2.75 mg/kg for 21 days, intraperitoneally), and parkinsonian treated with BBG (50 mg/kg for 15 days, intraperitoneally). Animals were subjected to behavioral tests and, at the end of the protocol, euthanized for brain collection for immunohistochemical and molecular analyses. P2X7 receptor blockade significantly protected the animals against deficits in volatile odor detection, locomotor activity (open field test), motor coordination (rotarod test), depressive-like behavior (sucrose preference test), and working memory (Y-maze test) induced by rotenone exposure. BBG prevented the loss of dopaminergic neurons in the substantia nigra and striatum, reduced neuroinflammation, and decreased the accumulation of total and phosphorylated α- synuclein. Additionally, BBG modulated autophagic and mitophagic pathways, as evidenced by reduced expression of proteins such as LC3 and PINK1. The effect of BBG occurred by decreasing the number of microglial P2X7 receptors. The results indicate that rotenone-induced microglial purinergic hyperactivity acts as a multifaceted pathogenic integration center, amplifying neuroinflammation, disrupting autophagic processes, and promoting the accumulation of pathogenic α-synuclein, thereby creating a vicious cycle that culminates in dopaminergic neuronal death. Thus, P2X7 receptor blockade emerges as a promising therapeutic approach for targeting multiple pathological pathways in PD. Tipo: Tese 2025-01-01T00:00:00Z