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Lectins isolated from Brazilian beans as markers of membrane glycoconjugates of human colon cancer cells

2026-09-03T15:48:43Z

Título: Lectins isolated from Brazilian beans as markers of membrane glycoconjugates of human colon cancer cells Autor(es): Teixeira, Alrieta Henrique; Pinto, Vicente de Paulo Teixeira; Teixeira, Edson Holanda; Carneiro, Victor Alves; Filho Cristino, Gerardo; Sampaio, Alexandre Holanda; Cavada, Benildo Sousa Abstract: In this preliminary study the carbohydrate-binding activity of 10 plant lectins from the Diocleinae subtribe Canavalia ensiformis (Con A), Canavalia brasiliensis (Con Br), Canavalia bonariensis (Con Bo), Canavalia grandiflora (Con Gr), Canavalia maritima (Con M), Dioclea grandiflora (DGL), Dioclea guianensis (Dgui), Dioclea virgata (Dvir), Dioclea violacea (Dvio) and Dioclea rostrata (Dros) was studied to determine their capacity to differentiate human colon carcinoma cell variants with respect to cell membrane glyco-receptors. Flow cytometry and confocal microscopy were used to identify the interactions between PITC-labeled lectin and cells. The lectins interacted with cells assayed in a dose dependent manner. In addition, the fluorescence spectra of Con Bo clearly differentiated the EB3 cells. Furthermore, confocal microscopy showed that Con Bo and Dvio seem to bind greatest to cell variants 8W. These preliminary data suggest a possible use of Diocleinae lectins as tools to identify quantitative and qualitative alterations in membrane glycoproteins during the oncogenic process, facilitating the diagnosis and improving the treatment. However, further experiments involving the use of appropriate controls are in progress. Tipo: Artigo de Periódico

Tecnologia educativa sobre sexualidade para adolescentes e jovens com transtornos do neurodesenvolvimento

2026-08-26T16:50:51Z

Título: Tecnologia educativa sobre sexualidade para adolescentes e jovens com transtornos do neurodesenvolvimento Autor(es): Mesquita, Edmara Rodrigues de Abstract: Sexuality is a fundamental dimension of human development and a right for everyone, including adolescents and young people with neurodevelopmental disorders (NDDs). However, the approach to this topic remains marked by stigma, gaps in professional training, and a scarcity of accessible educational resources. The aim of this study was to develop an educational tool regarding sexuality for adolescents and young people with NDDs. This was a methodological study with a qualitative approach, conducted in two stages. The first stage was a scoping review, carried out according to the Joanna Briggs Institute methodology, to identify existing educational tools on the subject. The second stage involved developing the educational tool, based on the *Guia de Orientação para o Desenvolvimento de Projetos* (Merino, 2016), with the participation of health and education professionals. The scoping review highlighted a lack of specific tools and the need for ongoing training for professionals, parents, and caregivers. Fourteen professionals—eight educators and six health workers—participated in the empirical stage; their perceptions revealed that the main challenges were insufficient professional training, sociocultural barriers, a lack of accessible educational materials, and resistance from families. The result was the development of an integrated educational package comprising a theoretical manual, an educational activity booklet, and four pedagogical dolls with anatomical sex characteristics designed for complementary use. These materials address topics such as the body, puberty, consent, privacy, relationships, self-care, and the prevention of vulnerabilities. The developed tool has the potential to improve sex education practices in health and educational settings, expanding the range of resources available to adolescents and young people with NDDs and contributing to the promotion of autonomy, social inclusion, and the protection of rights. Further studies are recommended to validate the tool with experts and the target audience (semantic validation), as well as to evaluate its usability and effectiveness across different application contexts. Tipo: Dissertação

Supervisão acadêmica do Programa Mais Médicos na Região Norte do estado do Ceará: desafios e potencialidades no processo pedagógico de capacitação e qualificação

2026-08-25T18:07:30Z

Título: Supervisão acadêmica do Programa Mais Médicos na Região Norte do estado do Ceará: desafios e potencialidades no processo pedagógico de capacitação e qualificação Autor(es): Arruda, Anderson Dias Abstract: The teaching-service activities developed by physicians in the Mais Médicos Program (PMM) are monitored and evaluated by an academic supervising physician, who must conduct at least one monthly in-person visit to the Basic Health Unit (UBS) where the physician is assigned. During this visit, the structural conditions of the unit, the work process, and the interaction of the supervised physician with the multidisciplinary team, the community, and municipal management are assessed. In this context, the general objective of the study was to analyze the perception of academic supervisors regarding the challenges and potential faced in the pedagogical process of training and qualification during the academic supervision of the Mais Médicos Program in the northern region of the state of Ceará. The methodology used was field research, characterized as analytical- descriptive with a qualitative approach. The research subjects were 14 academic supervising physicians who are part of the PMM and linked to the Visconde de Saboia School of Public Health in the northern region of the state of Ceará. The data were collected between August and September 2025. As a result of an in-depth reading of the interviews, four main categories emerged: identification of the interviewees, the pedagogical process of academic supervision, the facilitators and difficulties in the supervision process, and final considerations about the program. This process allowed us to understand how supervisors perceive their role and the challenges present in academic supervision within the context of primary health care. In general, the results show that academic supervision in the PMM plays a fundamental role in improving the quality of medical work in Primary Health Care. At the same time, the findings indicate the need to strengthen institutional strategies to support supervision, expanding opportunities for pedagogical training and improving mechanisms for monitoring and evaluating the activities carried out. Thus, academic supervision is consolidated as an important instrument to promote continuing education, strengthen the integration between teaching and service, and contribute to improving the quality of health care in the regions served by the program. In light of these findings, it is concluded that academic supervision within the Mais Médicos Program presents significant potential for the qualification of primary healthcare professionals, but demands structural, pedagogical, and institutional improvements. Investments in the organization of content, in the training of supervisors, and in strengthening the articulation between the different actors involved are essential to increase the effectiveness of this strategy and contribute to improving the quality of care provided to the population. Tipo: Dissertação

Análise da osteopromoção de membrana híbrida de celulose bacteriana e alginato com apatita de estrôncio em calvária de rato

2026-08-25T18:02:39Z

Título: Análise da osteopromoção de membrana híbrida de celulose bacteriana e alginato com apatita de estrôncio em calvária de rato Autor(es): Linhares, João Paulo Tavares Abstract: Recent studies have demonstrated a growing increase in the development of smart biomaterials, with release systems aimed at bone regeneration. Pure bacterial cellulose (BC) or its oxidized form (OxBC) is a biopolymer with favorable physicochemical properties, and its association with alginate (ALG) generates a stable hydrogel for the incorporation of strontium apatite (SrAp), a dual bioactive compound in bone remodeling. This study evaluated, in vivo, the osteopromotive effect of hybrid membranes based on BC or OxBC associated with ALG, with or without SrAp. Critical-size defects in rat calvaria were treated with one of the following experimental modalities: lyophilized implants of BC/ALG (G1), OxBC/ALG (G2), BC/ALG/SrAp (G3), OxBC/ALG/SrAp (G4), collagen membrane Lumina Coat® (C+), or blood clot (C−). Histological and histomorphometric analyses evaluated, at 1and 2 months, the parameters of presence of newly formed bone, connective tissue, and biomaterial. ANOVA followed by Tukey’s post hoc test considered p < 0.05 as indicative of significant differences between groups according to treatments and time points. New bone formation at 1 month did not exhibit differences; however, at 2 months, G2 was higher than C− and increased over time. Connective tissue filling at 1 month was lower in G1 and higher in G3 and G4; at 2 months, it was lower in G1 and G2 and higher in C+, G3, and G4, with reduction over time in C+. The amount of biomaterial at 1 and 2 months was more evident in G1 and G2, with persistent integrity in G1 and reduction in G2, whereas G3, G4, and C+ maintained low but stable frequency during the experimental period. The OxBC/ALG membrane was more promising, due to favorable osteopromotion, limited fibrogenesis, and low bioresorption. Although the incorporation of SrAp did not confer a consistent impact on bone regeneration under the evaluated experimental conditions and time period, the findings reinforce current challenges in biomaterials engineering related to the control of ionic release, carrier stability, and the bioactivity of strontium in hybrid systems. In this context, future investigations involving physicochemical modifications of biomaterials may broaden opportunities for the development of more efficient and predictable composites for bone regeneration. Tipo: Dissertação