http://repositorio.ufc.br/handle/riufc/389 2026-06-12T08:29:33Z 2026-06-12T08:29:33Z Mesquita, Amanda de Menezes http://repositorio.ufc.br/handle/riufc/86722 2026-06-11T17:35:27Z 2025-01-01T00:00:00Z

Título: Avaliação in vitro dos efeitos leishmanicida e imunomoduladores contra Leishmania infantum de lectinas isoladas de algas marinhas do litoral cearense Autor(es): Mesquita, Amanda de Menezes Abstract: Leishmaniasis, an infectious parasitic disease caused by protozoa of the genus Leishmania, is transmitted through the bite of infected female sandflies and primarily affects the mononuclear phagocytic system. Among its forms, visceral leishmaniasis (VL) is the most severe due to its high lethality. In Brazil, the drugs used for VL treatment include pentavalent antimonials and amphotericin B, which are associated with high toxicity and the emergence of resistant strains. Therefore, the search for new therapeutic agents is of great importance. This study aimed to investigate the immunomodulatory and leishmanicidal effects in vitro of lectins extracted from the red marine alga Solieria filiformis (Kützing) P.W. Gabrielson and the green alga Caulerpa cupressoides var. lycopodium against Leishmania infantum. After extraction and isolation of the lectins, cytotoxicity was evaluated in J774 macrophages. Subsequently, the leishmanicidal effect against the amastigote form of the parasite was assessed (24 and 48 hours post-infection) using concentrations of 5, 10, 25, 50, and 100 µg/mL. To understand the leishmanicidal mechanism of action, levels of nitric oxide (NO) and the pro- and anti-inflammatory cytokines IL-4, IL-6, IL-10, IL-12, and TNF-α were measured. The study also investigated the leishmanicidal effect of Solieria filiformis and Caulerpa cupressoides on promastigote forms. These proteins demonstrated significant leishmanicidal activity, reducing promastigote survival at different concentrations. LSf showed greater efficacy, with a reduction of up to 75.60% at 48 hours, while LCc achieved 55.68%. Regarding the leishmanicidal effect of LSf and LCc on amastigote forms in macrophages infected with L. infantum, LSf reduced the parasitic load more effectively than the positive control (GLU) at 24 and 48 hours, particularly at 100 μg/mL. NO production increased with LSf, especially at higher concentrations, while LCc induced a less pronounced increase in NO. As for cytokines, LSf elevated IL-12 and TNF-α only at specific concentrations and time points, whereas LCc significantly increased IL-10, indicating an anti-inflammatory effect. Both proteins induced IL-6 production in infected macrophages, with a more pronounced effect at 50 and 100 μg/mL. The data from this study suggest a promising immunomodulatory effect of lectins from red and green marine algae on L. infantum-infected macrophages, although further studies are needed to better elucidate the mechanisms of action of these compounds against this parasite.. Tipo: Dissertação

2025-01-01T00:00:00Z Rocha, Nyvea Maria de Holanda Maia http://repositorio.ufc.br/handle/riufc/86687 2026-06-10T17:11:47Z 2026-01-01T00:00:00Z

Título: Uso do jogo Brincatomia como ferramenta pedagógica jogo para o ensino de ciências nos anos finais do ensino fundamental Autor(es): Rocha, Nyvea Maria de Holanda Maia Abstract: Teaching the human body in middle school requires pedagogical strategies that make scientific content more accessible, contextualized, and meaningful for students. Topics related to the morphology and functioning of organ systems, although closely connected to students’ everyday experiences, may become abstract when addressed only through expository practices centered on memorization. In this context, educational games emerge as methodological possibilities capable of promoting student participation, peer interaction, and the review of concepts in a playful manner guided by pedagogical intentionality. This study evaluated the pedagogical effectiveness of the board game BRINCATOMIA as a teaching resource for Science education on the human body in the 6th grade of middle school. This is a mixed- methods study with a quasi-experimental design, developed through stages of game design, validation, and classroom application. The validation process involved specialists from different fields, who evaluated aspects related to clarity, relevance, language, appearance, functionality, and pedagogical applicability of the material. The classroom application was conducted with two classes organized into a control group, which received dialogued expository lessons, and an intervention group, which received dialogued expository lessons associated with the use of BRINCATOMIA. Conceptual learning was examined through pre- and post-tests, with within-group and between-group comparisons. To further understand the students’ experience, structured interviews were conducted with volunteer students who participated in the intervention, and their responses were analyzed qualitatively. The results indicated statistically significant improvement in both groups, with superior post-test performance in the intervention group, suggesting an additional effect of the game when integrated into classroom instruction. In the qualitative strand, students associated the BRINCATOMIA experience with advantages related to collaborative learning, peer interaction, communication, motivation, and the perception of “learning while playing.” Conversely, operational challenges emerged, including noise, distraction, isolated conflicts, and limitations of physical space, indicating the need for agreed-upon rules, classroom organization, and teacher mediation to sustain pedagogical intentionality. It is concluded that BRINCATOMIA, when articulated with teaching practice, can enhance immediate learning gains and foster more participatory classroom conditions, although its effects depend on planning, pedagogical management, and adaptation to the school context. This research contributes to the teaching of morphofunctional sciences by proposing, validating, and applying a teaching strategy aimed at supporting the learning of knowledge about the morphology and functioning of the human body from Basic Education onward, expanding the possibilities of connection between scientific knowledge, pedagogical practice, and school education. Tipo: Dissertação

2026-01-01T00:00:00Z Souza, João Paulo Fernandes de http://repositorio.ufc.br/handle/riufc/86685 2026-06-10T14:43:06Z 2026-01-01T00:00:00Z

Título: Estudo morfofuncional de fístulas arteriovenosas: uma abordagem com ultrassom ecodoppler Autor(es): Souza, João Paulo Fernandes de Abstract: Chronic Kidney Disease represents a major public health problem, and hemodialysis remains one of the main renal replacement therapies in the advanced stages of the disease. In this context, the arteriovenous fistula is considered the vascular access of choice, although it is subject to morphological and hemodynamic changes that may compromise its function. This study aimed to systematize the morphofunctional findings observed in ultrasound images of arteriovenous fistulas in individuals with Chronic Kidney Disease. This is an observational, cross-sectional, descriptive, and analytical study, based on the acquisition and analysis of ultrasound images of arteriovenous fistulas performed between November 2024 and April 2025 at the Hemodialysis Clinic of Hospital de S.o Francisco do Porto, Portugal. Autologous and prosthetic arteriovenous fistulas were evaluated considering morphological, structural, and hemodynamic aspects through B-mode, color Doppler, and spectral Doppler. The findings showed different ultrasonographic patterns, including fistulas with preserved flow, as well as alterations compatible with aneurysm, stenosis in a prosthetic segment, inflow stenosis, outflow stenosis, and thrombosis. These results show that Doppler ultrasound can identify structural changes and relevant hemodynamic repercussions in vascular access. It is concluded that ultrasound is a useful tool in the morphofunctional assessment of arteriovenous fistulas, contributing to the identification of patterns of normality and dysfunction, as well as to clinical follow-up and decision-making in the care of patients undergoing hemodialysis. Tipo: Tese

2026-01-01T00:00:00Z Mont’Alverne, Ricardo Everton Dias http://repositorio.ufc.br/handle/riufc/86620 2026-06-08T12:25:40Z 2025-01-01T00:00:00Z

Título: Correlação da incontinência fecal com parâmetros clínicos, funcionais e anatômicos de pacientes do sexo feminino submetidas à secção da musculatura esfincteriana lisa pós fistulotomia anal e esfincterotomia anal Autor(es): Mont’Alverne, Ricardo Everton Dias Abstract: Introduction: Anal fistula and anal fissure are benign diseases affecting the anal canal; however, surgical treatment may be required, often involving division of the internal sphincter muscle. As a consequence, patients may experience sequelae that significantly impact their quality of life, such as fecal incontinence, with female patients being particularly susceptible. Methodology: Sixty-three female patients who underwent internal sphincter muscle division through anal fistulotomy (30 patients) or anal sphincterotomy (33 patients) were evaluated. Clinical assessment was performed using a fecal incontinence score, functional assessment by anorectal manometry, and anatomical assessment by 3D endoanal ultrasound. Results: Overall, 46% (29/63) of patients reported symptoms of fecal incontinence after internal anal sphincter division, with 18 (60%) in group I and 16 (48%) in group II. However, the severity of symptoms was similar between groups, with mean scores of 1.6 (0–8) in group I and 2 (0–7) in group II. Resting pressure decreased in both groups and was similar between them postoperatively. The longitudinal length and percentage of the internal sphincter muscle divided were greater in group I. The longitudinal length of the anterior external anal sphincter and the distance between the external anal sphincter and puborectalis muscle were similar in both groups. However, the gap length—the distance between the proximal edge of the external anal sphincter and the distal edge of the puborectalis—was significantly greater in group II. Conclusion: Sphincter division surgery, regardless of the operative technique, is associated with a high rate of fecal incontinence, although the symptom severity remains low. The length and percentage of smooth muscle divided were smaller in patients who underwent anal sphincterotomy. Nevertheless, the rate of fecal incontinence was similar between the two groups. This finding may be explained by the longer gap observed in this group, leading to greater asymmetry of the anal canal. Tipo: Dissertação

2025-01-01T00:00:00Z