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Avaliação dos efeitos da ozonioterapia na dor, trismo e consumo medicamentoso após cirurgia de terceiro molar: ensaio clínico randomizado e triplo cego

2026-08-18T15:52:25Z

Título: Avaliação dos efeitos da ozonioterapia na dor, trismo e consumo medicamentoso após cirurgia de terceiro molar: ensaio clínico randomizado e triplo cego Autor(es): Paiva, Calebe Lamonier De Oliveira Costa Abstract: Third molar extraction is a common surgical procedure, often associated with postoperative complications such as pain, swelling and trismus. Ozone therapy has been proposed as a promising adjunctive approach to modulate the inflammatory response and accelerate healing. The aim of the present study was to evaluate the efficacy and safety of ozone therapy as an adjunct in the postoperative management of third molar extractions, compared with the conventional surgical technique. This was a randomized, triple-blind, split-mouth clinical trial conducted in 20 patients undergoing bilateral mandibular third molar extraction. One side was treated with ozone therapy in various forms (ozone gas, ozonated water and ozonated oil—Ozone Group), while the contralateral side received placebo interventions (Placebo Group). Outcomes assessed included self-reported pain intensity (visual analog scale, VAS), maximal interincisal opening and the quantity of analgesic medication consumed at 24 h, 72 h and 7 days postoperatively. Data were analyzed using Wilcoxon and Friedman/Dunn tests (p < 0.05). There was no statistically significant difference in surgical time between groups (p = 0.407). The Ozone Group reported lower pain perception at all time points (p = 0.037) and reduced analgesic consumption (7.75 ± 10.79 tablets) compared with the Placebo Group (10.40 ± 10.52 tablets) (p = 0.001). Maximal interincisal opening was significantly greater in the Ozone Group (51.75 ± 6.88 mm) versus the Placebo Group (50.00 ± 6.23 mm) on postoperative day 7 (p = 0.049). No complications such as infection or alveolitis were observed in either group. Ozone therapy proved to be an effective and safe adjunctive approach for reducing postoperative pain, decreasing analgesic consumption and improving mouth opening after third molar extractions. Although the sample size was limited, the results indicate a potential clinical benefit of ozone therapy. Tipo: Dissertação

Atividades biológicas dos derivados semissintéticos do triterpeno cl-1 isolado do combretum leprosum mart. em modelos experimentais: uma revisão de escopo.

2026-08-18T15:48:09Z

Título: Atividades biológicas dos derivados semissintéticos do triterpeno cl-1 isolado do combretum leprosum mart. em modelos experimentais: uma revisão de escopo. Autor(es): Leão, Rebeca Moita Abstract: Natural products represent an important source for the identification of bioactive molecules, particularly when combined with structural modification strategies such as semisynthesis. In this context, the triterpene CL-1, isolated from Combretum leprosum Mart., has attracted considerable interest due to the pharmacological potential of its semisynthetic derivatives. This scoping review aimed to analyze the available evidence regarding the biological activities of CL-1 semisynthetic derivatives in experimental models. The review was conducted in accordance with the recommendations of the Joanna Briggs Institute and the PRISMA-ScR guidelines. The research question was structured using the Population–Concept–Context framework, in which the population consisted of CL-1 semisynthetic derivatives, the concept encompassed biological activities and pharmacological outcomes, and the context included in vitro, in vivo, and/or in silico experimental models. The search covered all publications available up to February 14, 2026, without language restrictions. Following the selection process, seven studies were included in the final sample. The results revealed a variety of biological activities attributed to CL-1 semisynthetic derivatives, including antiparasitic, antiplasmodial, cytotoxic, antinociceptive, osteogenic, osteoprotective, and antiresorptive effects. The findings demonstrated that structural modifications of the parent molecule were capable of modulating its biological behavior, enhancing, reducing, or redirecting its activity depending on the derivative evaluated and the experimental model employed. Among the investigated compounds, CL-P2 and CL-P2A stood out due to their recurrence in the most recent studies and their favorable results related to biological safety, inflammatory pain modulation, and bone metabolism. Evidence regarding bone formation, mineralization, the RANKL/OPG axis, and the reduction of experimental bone loss highlights a promising field for future investigations, particularly in conditions associated with impaired bone remodeling. However, the available studies remain predominantly preclinical and exhibit methodological heterogeneity regarding experimental models, doses, routes of administration, and assessed outcomes. It can be concluded that CL-1 semisynthetic derivatives possess relevant biological potential, especially concerning bone metabolism, but additional studies are still required to elucidate their mechanisms of action, assess selectivity, determine long-term safety, and validate their effects in more robust experimental models. Tipo: Dissertação

Desenvolvimento de um gel contendo trans-cinamaldeído e avaliação da atividade antibacteriana sobre Enterococcus faecalis: um estudo in vitro e ex vivo

2026-08-17T18:53:20Z

Título: Desenvolvimento de um gel contendo trans-cinamaldeído e avaliação da atividade antibacteriana sobre Enterococcus faecalis: um estudo in vitro e ex vivo Autor(es): Maia Júnior, Virgílio Mendes Abstract: Eliminating microorganisms from the root canal system (RCS) is a fundamental step for the success of any endodontic procedure. Chemomechanical preparation and the administration of intracanal medication (IM) significantly reduce the microbial load and its metabolic byproducts. The aim of this study was to develop an IM containing trans-cinnamaldehyde (GelTC-Paste), evaluate, in vitro and ex vivo, its antibacterial effect on Enterococcus faecalis(ATCC 29212), and assess the cytotoxicity of trans-cinnamaldehyde (TC) in L-929 fibroblasts (ATCC CCL-1). In the in vitro study, concentrations of 0.1%, 0.5%, 1.0%, 2.5%, and 5.0% of GelTC-Paste were tested. For the ex vivo experiment, 64 single-rooted human permanent teeth were used. The specimens were decoronated, instrumented, and contaminated with E. faecalis for 21 days at 37 ºC, except for the control group. They were then flooded with saline solution, and absorbent paper points were inserted for 1 minute and immersed in Eppendorf tubes containing 1 ml of saline solution. Aliquots of 100 μl were streaked onto brain heart infusion (BHI) plates and incubated for 24 hours at 37 ºC. The specimens were randomly divided into four groups: Saline (n = 6), 5% GelTC-Paste (n = 10), Calcium Hydroxide Paste (n = 10), and Control Group (n = 6). After 7 and 14 days, the IMs were removed, and bacterial growth was measured by counting colony-forming units (CFU/ml). For statistical analysis, analysis of variance (ANOVA) and Tukey's post-hoc test were used. The GelTC-Paste demonstrated structural stability under stress and maintenance of its physicochemical characteristics. The inhibition halo measurements showed an average of 12.2 (0.1%), 14.5 (0.5%), 18.25 (1%), 24.85 (2.5%), and 27.2 (5%) for each GelTC-Paste concentration. The gel base did not demonstrate activity, and pure TC showed the largest inhibition halo (average of 36.9 mm). In the MTT assay, TC maintained cell viability equal to the control group up to a concentration of 15.6 μg/mL. In the ex vivo study, the 5% GelTCPaste eliminated 41.9% of the intracanal biofilm of E. faecalis after 7 days, compared to 14% for the calcium hydroxide paste. After 14 days, there was a 34.9% reduction in biofilm in the 5% GelTC-Paste group, surpassing the 19.6% reduction achieved by Ca(OH)2. The findings of this study indicate that the 5% GelTC-Paste has an antibacterial effect on E. faecalis biofilms when compared to calcium hydroxide, suggesting potential for application as an IM in cases of resistant infections. Tipo: Dissertação

Atividade antifúngica do trans-cinamaldeído contra biofilme de candida albicans formado em resina acrílica termopolimerizável

2026-08-12T19:21:08Z

Título: Atividade antifúngica do trans-cinamaldeído contra biofilme de candida albicans formado em resina acrílica termopolimerizável Autor(es): Cunha, Abrahão Lincoln Alves Abstract: Dental prosthesis stomatitis (DPS) is a multifactorial inflammatory condition frequently found in patients who wear removable full dentures, with the microbial biofilm that develops on the acrylic resin being a key factor in its onset. Biofilms are complex communities that can consist of different species of bacteria and fungi, with Candida albicans being the primary microorganism responsible for the development of DPS. Cleaning agents are recommended to minimize biofilm formation; however, they can cause adverse effects, such as mucosal irritation, alter the physical properties of the resin, and promote resistance among microorganisms. Compounds derived from medicinal plants are a promising alternative for the formulation of new drugs; trans-cinnamaldehyde stands out as the major compound present in the bark of Cinnamomum zeylanicum, an option due to its broad antimicrobial activity. The objective of this study was to evaluate the antifungal and antibiofilm activity of transcinnamaldehyde against Candida albicans biofilm induced on the surface of thermopolymerizable acrylic resins. The concentration of interest was selected based on the minimum inhibitory concentration (MIC). Antimicrobial activity was also evaluated by quantifying the biofilm and assessing the viability of fungal cells present in the biofilm using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay. For qualitative analysis of the biofilm present on the surfaces of the acrylic resin, samples o f the biofilm formed—treated and untreated with trans-cinnamaldehyde—had their surfaces photographed using atomic force microscopy (AFM). The minimum inhibitory concentration was 0.078 mg/mL, and the minimum fungicidal concentration was 0.156 mg/mL. In biofilm inhibition assays, the compound significantly reduced biofilm viability, achieving inhibition rates exceeding 90% at concentrations of 4xMIC, 8xMIC, and 16xMIC. In acrylic resin test specimens, a progressive decrease in biofilm formation was observed as concentration and exposure time increased. Atomic force microscopy analyses revealed morphological changes in C. albicans hyphae, especially at the highest concentrations tested. Furthermore, transcinnamaldehyde exhibited low cytotoxicity in L929 fibroblasts, maintaining cell viability at concentrations below 250 µg/mL, demonstrating promising potential as a therapeutic alternative for the management of prosthetic stomatitis associated with fungal biofilms composed of C. albicans. Tipo: Dissertação