Estudo do potencial citotóxico dos metabólitos secundários isolados do fungo marinho Paecilomyces lilacinus recuperado de sedimentos da costa cearense

Abstract

The bioprospection of cytotoxic secondary metabolites produced by Paecilomyces lilacinus (fungal strain BRF053) recovered from sediments collected at Pecém beach, Ceará state, was investigated. First, the production of compounds by the fungal strain was performed by culturing the microorganism in potato-dextrose broth (PDB) for 7, 14, 21 and 28 days. Both HPLC analysis and cytotoxic activity (tumor cell line HCT-116) of the extracts from each period of culture revealed 14 days as the optimum time for producing cytotoxic compounds (growth inhibition 85.7 %). Bioguided fractionation of the organic extract from the fungus cultured in large scale under the same conditions (14 days) allowed the isolation of the steroids wortimannin and 11-deacetoxywortimannin, both new compounds in Paecilomyces. These compounds were isolated by chromatography column and HPLC, and their st ructures were elucidated by spectrometric methods (HRMS, 1H, 13C NMR, and IR) besides comparison with literature data. Dereplication of the extract by LC-MS suggested the presence of cyclosinefungin, stanna A, virescenoside-D, (+)-esclerotiorin, 2'-Amino-2'-deoxiguanosine, fumaramidimicin e deoxynivalenol, all of the new compounds in Paecilomyces.