Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/9472
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dc.contributor.authorGuimarães Filho, Artur-
dc.contributor.authorCunha, Rodrigo Maranguape Silva da-
dc.contributor.authorVasconcelos, Paulo Roberto Leitão de-
dc.contributor.authorGuimarães, Sergio Botelho-
dc.date.accessioned2014-10-16T16:18:19Z-
dc.date.available2014-10-16T16:18:19Z-
dc.date.issued2014-06-
dc.identifier.citationGUIMARÃES FILHO, A. et al. Glutamine and ornithine alpha-ketoglutarate supplementation on malate dehydrogenases expression in hepatectomized rats. Acta Cirúrgica Brasileira, São Paulo, v. 29, n. 6, jun., 2014.pt_BR
dc.identifier.issn1678-2674 Online-
dc.identifier.urihttp://www.repositorio.ufc.br/handle/riufc/9472-
dc.description.abstractPURPOSE: To evaluate the relative gene expression (RGE) of cytosolic (MDH1) and mitochondrial (MDH2) malate dehydrogenases enzymes in partially hepatectomized rats after glutamine (GLN) or ornithine alpha-ketoglutarate (OKG) suplementation. METHODS: One-hundred and eight male Wistar rats were randomly distributed into six groups (n=18): CCaL, GLNL and OKGL and fed calcium caseinate (CCa), GLN and OKG, 0.5g/Kg by gavage, 30 minutes before laparotomy. CCaH, GLNH and OKGH groups were likewise fed 30 minutes before 70% partial hepatectomy. Blood and liver samples were collected three, seven and 14 days after laparotomy/hepatectomy for quantification of MDH1/MDH2 enzymes using the real-time polymerase chain reaction (PCR) methodology. Relative enzymes expression was calculated by the 2-ΔΔCT method using the threshold cycle (CT) value for normalization.RESULTS: MDH1/MDH2 RGE was not different in hepatectomized rats treated with OKG compared to rats treated with CCa. However, MDH1/MDH2 RGE was greater on days 3 (321:1/26.48:1) and 7 (2.12:1/2.48:1) while MDH2 RGE was greater on day 14 (7.79:1) in hepatectomized rats treated with GLN compared to control animals. CONCLUSION: Glutamine has beneficial effects in liver regeneration in rats by promoting an up-regulation of the MDH1 and MDH2 relative gene expression.pt_BR
dc.language.isoenpt_BR
dc.publisherActa Cirúrgica Brasileirapt_BR
dc.subjectGlutaminapt_BR
dc.subjectHepatectomiapt_BR
dc.subjectRegeneração Hepáticapt_BR
dc.titleGlutamine and ornithine alpha-ketoglutarate supplementation on malate dehydrogenases expression in hepatectomized ratspt_BR
dc.typeArtigo de Periódicopt_BR
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