Suscetibilidade de Acinetobacter baumannii multidroga-resistente à terapia fotodinâmica antimicrobiana e à ozonioterapia

Abstract

Environments with a risk of biological infection, as is the case in most hospitals, are considered hospitable. The numbers of hospitalized people have grown all over the world, mainly associated with multidrug-resistant microorganisms (OM), representing a public health problem by increasing mortality rates, morbidity, the length of stay of patients in the hospital and treatment costs. The development of alternative antimicrobial therapies for the treatment of these OM is highlighted by the need to reduce problems and health problems. In this sense, Antimicrobial Photodynamic Therapy (APT) and Ozone Therapy (OT) appear as alternative and/or adjuvant treatments to the use of conventional antibiotics. The study aimed to evaluate the susceptibility of A. baumannii strains multiresistant to pediatric drugs of a patient in the intensive care unit of Hospital Santa Casa de Misericórdia of Sobral to antimicrobial photodynamic therapy and ozone therapy. Ortho-toluidine blue prepared with distilled water or ozonized water was used as a minimal sensitizer and the inhibitory concentration and the minimum bactericidal concentration of these sensitizers under the clinical isolate of A. baumannii was initially tested. Additionally, a model of biofilm formation with 24h, 72h and 120h of formation on sterile PVC disks, with 6mm in diameter, was used. Groups treated with light only (S-L+), dye only (S+L-) and TFA confirmed (S+L+) were tested. These same groups were tested with the sensitizer prepared with ozonated water, namely with light only (S-L+OZ), with the dye only (S+L-OZ) and TFA expressed (S+L+OZ). The irradiation time was 3 minutes. Three replicates were performed for each experiment. The results confirmed that A. baumannii biofilms with 24h, 72h and 120h of formation were susceptible to TFA and TFA + OZ (p<0.05), and the TFA group without association with OZ was the one that presented the better results. It is concluded that TFA instructed or associated with OZ has an inhibitory effect on multidrug-resistant A. baumannii biofilms.