Clonagem da região codificadora do gene VuGOX1-like de Vigna Unguiculata [L.] Walp

Abstract

The cowpea bean (Vigna unguiculata [L.] Walp) is a crop of great importance in several countries of the tropical and subtropical region thanks to its nutritional aspects and its resilience to drought. This plant species can be affected by a diversity of biotic and abiotic stresses, aggravated by the drastic climate changes that the planet has been going through. Thus, it is important to ensure means to preserve the cultivation of V. unguiculata given its social and economic importance to the world. In relation to this, plants can respond to environmental stresses in several ways and one of them is through the expression of specific genes that generate metabolic responses with the function of promoting resistance mechanisms against the stress to which they are being submitted. The VuGOX1-like gene encodes an important peroxisome enzyme called Glycolate Oxidase 1 (GOX1), which is responsible for the release of hydrogen peroxide (H2O2) during the photorespiratory process. H2O2 is a molecule that plays a key role in signaling defense processes and programmed cell death in plants. Previous studies have shown that GOX1 is associated with resistance against biotic and abiotic stresses. In the present work, by using molecular biology techniques, the coding region of the VuGOX1-like gene was cloned and confirmed in the input vector pENTR11-DUAL SELECTION VECTOR. The generated construct can be used for subcloning into expression vectors for the functional study of the VuGOX1-like gene in future assays.