Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/6939
Title in Portuguese: Phytochemical study guided by the myorelaxant activity of the crude extract, fractions and constituent from stem bark of Hymenaea courbaril L.
Author: Bezerra, Gabrieli Penha
Góis, Roberto Wagner da Silva
Brito, TeresinhaSilvade
Lima, Francisco José Batista de
Bandeira, Mary Anne Medeiros
Romero, Nirla Rodrigues
Magalhães, Pedro Jorge Caldas
Santiago, Gilvandete Maria Pinheiro
Keywords: Antioxidantes
Hymenaea
Issue Date: Aug-2013
Publisher: Journal of Ethnopharmacology
Citation: BEZERRA, G. P. et al. Phytochemical study guide by the myorelaxant activity of the crude extract, fractions and constituent from stem barkof Hymenaea courbaril L. Journal of Ethnopharmacology, Lausanne, v. 149, n. 1, p. 62-69, ago. 2013.
Abstract: Ethnopharmacological relevance:Hymenaeacourbaril L. (Caesalpinoideae)isusedinBrazilianfolkmedicine totreatanemia,kidneyproblems,sorethroatandotherdysfunctionsoftherespiratorysystem,suchas bronchitisandasthma,althoughsuchpropertiesareyettobescientificallyvalidated. Aimofthestudy: Inordertogiveascientific basistosupportthetraditionaluseof Hymenaea courbaril, this study wasdesignedtoevaluateantioxidant,myorelaxantandanti-inflammatorypropertiesoftheethanol extractfromstembarkanditsfractions.Themyorelaxanteffectofastilbin,a flavonoidisolatedfromthe bioactiveethylacetatefraction(EAF),hasalsobeenevaluated. Materialandmethods: Inthepresentstudyethanolextractfromstembark(EEHC)andfractionswereanalyzed usingbioassay-guidedfractionation.Thefollowingactivitieswereinvestigated:antioxidantby2,2-diphenyl-1- picrylhydrazyl(DPPH)assay,myorelaxant onrattrachealsmoothmuscle,andanti-inflammatoryusing ovalbumin-inducedleukocytosisandairwayhyperresponsivenessinrats. Results: The resultsofthepresentinvestigationshowthatthewholeextractof Hymenaeacourbaril and someof its fractionsstronglyscavengedDPPHradical.Theextract showedmyorelaxantactivityonrattrachea,beingEAF its highestefficientfraction.Bio-guidedstudyallowedtheisolationofastilbin,awell-known flavonoid.The activityinducedbythiscompoundindicatesthatitmaybe partlyresponsibleforthemyorelaxanteffectofEAF. EAF reducedcontractionsthatdependedondivalentcationinflowthroughvoltage-operatedCa2+ channels (VOCCs) orreceptor-operatedCa2+ channels (ROCCs),butitwasmorepotenttoinhibitVOCC-thanROCC- dependent contractioninducedbyCa2+ additioninACh-enrichedCa2+-freemedium.Oralpretreatmentof antigen-challengedanimalswithEAFpreventedairwayhyperresponsivenessonKCl-inducedcontractionand reducedthenumberoftotalwhitecells,particularlyeosinophilsandneutrophilsinbronchoalveolarlavage. Conclusions: This studyprovidedscientific basisthat Hymenaea courbaril presentspotentialantioxidant, myorelaxantandanti-inflammatoryactions,whichsupportitsuseinfolkmedicinetotreatinflammatory airwaydiseases.
URI: http://www.repositorio.ufc.br/handle/riufc/6939
ISSN: 0378-8741
Appears in Collections:DFAR - Artigos publicados em revistas científicas

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