Use este identificador para citar ou linkar para este item: http://repositorio.ufc.br/handle/riufc/59812
Tipo: Artigo de Evento
Título: Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
Título em inglês: Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
Autor(es): Kerkis, Alexandre
Kerkis, Irina
Rádis-Baptista, Gandhi
Oliveira, Eduardo B.
Vianna-Morgante, Angela M.
Pereira, Lygia V.
Yamane, Tetsuo
Palavras-chave: Cromossomas;Dna;Peptídeos
Data do documento: 2004
Instituição/Editor/Publicador: The FASEB Journal express
Citação: KERKIS, Alexandre; KERKIS, Irina; RÁDIS-BAPTISTA, Ghandi; OLIVEIRA, Eduardo B.; VIANNA-MORGANTE, Angela M.; PEREIRA, Lygia V.; YAMANE, Tetsuo. Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus The FASEB Journal express, United States, art. 10.1096/fj.03-1459fje. 2004.
Abstract: Herein we report that crotamine, a small lysine- and cysteine-rich protein from the venom of the South American rattlesnake, can rapidly penetrate into different cell types and mouse blastocysts in vitro. In vivo, crotamine strongly labels cells from mouse bone marrow and spleen and from peritoneal liquid, as shown by fluorescent confocal laser-scanning microscopy. Nuclear localization of crotamine was observed in both fixed and unfixed cells. In the cytoplasm, crotamine specifically associates with centrosomes and thus allows us to follow the process of centriole duplication and separation. In the nucleus, it binds to the chromosomes at S/G2 phase, when centrioles start dividing. Moreover, crotamine appears as a marker of actively proliferating cells, as shown by 5-BrdU cell-proliferation assay. Crotamine in the micromolar range proved nontoxic to any of the cell cultures tested and did not affect the pluripotency of ES cells or the development of mouse embryos
URI: http://www.repositorio.ufc.br/handle/riufc/59812
ISSN: 0892-6638
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