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Title in Portuguese: Mangiferin ameliorates 6-hydroxydopamine- induced cytotoxicity and oxidative stress in ketamine model of schizophrenia
Author: Rao, Vietla S.
Carvalho, Ana C. S.
Trevisan, Maria Teresa S.
Andrade, Geanne M.
Nobre Júnior, Hélio Vitoriano
Moraes Filho, Manoel Odorico de
Magalhães, Hemerson Iury
Morais, Talita C.
Santos, Flavia A.
Keywords: Citoproteção
Estresse Oxidativo
Issue Date: 2012
Publisher: Pharmacological reports
Citation: RAO, V. S. et al. Mangiferin ameliorates 6-hydroxydopamine-induced cytotoxicity and oxidative stress in ketamine model of schizophrenia. Pharmacological reports, v. 64, n. 4, p. 848-856, 2012.
Abstract: Mangiferin ameliorates 6-hydroxydopamine- induced cytotoxicity and oxidative stress in ketamine model of schizophrenia Vietla S. Rao 1 , Ana C. Carvalho 1 , Maria Teresa S. Trevisan 2 , Geanne M. Andrade 1 , Helio V. Nobre Júnior 1 , Manoel O. Moraes 1 , Hemerson I. Magalhães H. Iury 1 , Talita C. Morais 1 , Flavia A. Santos 1 1 Department of Physiology and Pharmacology, Federal University of Ceará, Cel Nunes de Melo-1127, Caixa Postal-3157, 60430-270 Fortaleza, CE, Brazil 2 Department of Organic and Inorganic Chemistry, Federal University of Ceara, Fortaleza, CE, Brazil Correspondence: Vietla S. Rao, e-mail: ; Abstract: Background: Accumulating evidence indicates that mangiferin (MGF), a natural xanthone, by virtue of its antioxidant and anti- inflammatory properties is neuroprotective. Here we sought to verify the cytoprotective role of MGF on cultured rat primary mesen- cephalic cells exposed to 6-hydroxydopamine (6-OHDA) in vitro , and the MGFs anti-inflammatory potential in mouse model of ketamine-induced schizophrenia in vivo . Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliu m bromide (MTT)-assay was performed to measure cell viabili ty in mesen- cephalic cell cultures exposed to neurotoxin (6-OHDA, 40 μM ). Schizophrenia was induced in mice by ketamine (50 mg/kg, ip , twice a day, for 7 days). The treatment effects of MGF (50 mg/kg, po , for 7 days) were verified on locomotor behavioral changes i n open-field test, and on the oxidant stress-related increase in lipid-peroxi dation (malondialdehyde) and interleukin-6 (IL-6) levels in brain tissues. Results: MGF (10–100 μM) produced no per se effect on cell viability as measured by MTT assay, but significantly prevented the 6-OHDA-induced cell death in a concentration-dependent manner. Acridine orange/ethidium bromide (AO/EtBr) staining con - firmed the absence of 6-OHDA-induced morphological changes characteristic of apoptosis/necrosis. In open-field test, ketamine- induced impaired locomotor activity and behavioral changes such as grooming and stereotyped but not rearing were effectively ame - liorated by MGF pretreatment. Also, ketamine-associated increase in brain tissue levels of IL-6 and MDA were significantly lowered in MGF-pretreated mice. Conclusion: Mangiferin has a neurocytoprotective role related, at least in part, to an antioxidant and anti-inflammatory mechanism, which could be explored for more effective therapies of schizophrenia and other neurodegenerative diseases.
metadata.dc.type: Artigo de Periódico
ISSN: 1734-1140
Appears in Collections:DFIFA - Artigos publicados em revista científica

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