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|Title in Portuguese:||Mangiferin ameliorates 6-hydroxydopamine- induced cytotoxicity and oxidative stress in ketamine model of schizophrenia|
|Author:||Rao, Vietla S.|
Carvalho, Ana C. S.
Trevisan, Maria Teresa S.
Andrade, Geanne M.
Nobre Júnior, Hélio Vitoriano
Moraes Filho, Manoel Odorico de
Magalhães, Hemerson Iury
Morais, Talita C.
Santos, Flavia A.
|Citation:||RAO, V. S. et al. Mangiferin ameliorates 6-hydroxydopamine-induced cytotoxicity and oxidative stress in ketamine model of schizophrenia. Pharmacological reports, v. 64, n. 4, p. 848-856, 2012.|
|Abstract:||Mangiferin ameliorates 6-hydroxydopamine- induced cytotoxicity and oxidative stress in ketamine model of schizophrenia Vietla S. Rao 1 , Ana C. Carvalho 1 , Maria Teresa S. Trevisan 2 , Geanne M. Andrade 1 , Helio V. Nobre Júnior 1 , Manoel O. Moraes 1 , Hemerson I. Magalhães H. Iury 1 , Talita C. Morais 1 , Flavia A. Santos 1 1 Department of Physiology and Pharmacology, Federal University of Ceará, Cel Nunes de Melo-1127, Caixa Postal-3157, 60430-270 Fortaleza, CE, Brazil 2 Department of Organic and Inorganic Chemistry, Federal University of Ceara, Fortaleza, CE, Brazil Correspondence: Vietla S. Rao, e-mail: firstname.lastname@example.org ; email@example.com Abstract: Background: Accumulating evidence indicates that mangiferin (MGF), a natural xanthone, by virtue of its antioxidant and anti- inflammatory properties is neuroprotective. Here we sought to verify the cytoprotective role of MGF on cultured rat primary mesen- cephalic cells exposed to 6-hydroxydopamine (6-OHDA) in vitro , and the MGFs anti-inflammatory potential in mouse model of ketamine-induced schizophrenia in vivo . Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliu m bromide (MTT)-assay was performed to measure cell viabili ty in mesen- cephalic cell cultures exposed to neurotoxin (6-OHDA, 40 μM ). Schizophrenia was induced in mice by ketamine (50 mg/kg, ip , twice a day, for 7 days). The treatment effects of MGF (50 mg/kg, po , for 7 days) were verified on locomotor behavioral changes i n open-field test, and on the oxidant stress-related increase in lipid-peroxi dation (malondialdehyde) and interleukin-6 (IL-6) levels in brain tissues. Results: MGF (10–100 μM) produced no per se effect on cell viability as measured by MTT assay, but significantly prevented the 6-OHDA-induced cell death in a concentration-dependent manner. Acridine orange/ethidium bromide (AO/EtBr) staining con - firmed the absence of 6-OHDA-induced morphological changes characteristic of apoptosis/necrosis. In open-field test, ketamine- induced impaired locomotor activity and behavioral changes such as grooming and stereotyped but not rearing were effectively ame - liorated by MGF pretreatment. Also, ketamine-associated increase in brain tissue levels of IL-6 and MDA were significantly lowered in MGF-pretreated mice. Conclusion: Mangiferin has a neurocytoprotective role related, at least in part, to an antioxidant and anti-inflammatory mechanism, which could be explored for more effective therapies of schizophrenia and other neurodegenerative diseases.|
|metadata.dc.type:||Artigo de Periódico|
|Appears in Collections:||DFIFA - Artigos publicados em revista científica|
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