Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/5698
Title in Portuguese: Preconditioning with a novel metallopharmaceutical NO donor in anesthetized rats subjected to brain ischemia/reperfusion
Author: Oriá, Reinaldo Barreto
Lopes, Luiz Gonzaga de França
Brito, Gerly Anne de Castro
Santos, Armenio Aguiar dos
Vasconcelos, Raquel Cavalcante de
Silva, Francisco Ordelei Nascimento da
Nobrega, Beatrice Nuto
Silva, Moisés Tolentino Bento da
Vasconcelos, Paulo Roberto Leitão de
Campelo, Marcio Wilker Soares
Keywords: Óxido Nítrico
Rutênio
Issue Date: Aug-2012
Publisher: Neurochemical Research
Citation: CAMPELO, M. W. S. et al. Preconditioning with a novel metallopharmaceutical no donor in anesthetized rats subjected to brain ischemia/reperfusion. Neurochemical Research, New York, US, v. 37, n. 4, p. 749-758, ago. 2012.
Abstract: Rut-bpy is a novel nitrosyl–ruthenium complex releasing NO into the vascular system. We evaluated the effect of Rut-bpy (100 mg/kg) on a rat model of brain stroke. Forty rats were assigned to four groups (Saline solution [SS], Rut-bpy, SS?ischemia–reperfusion [SS?I/ R] and Rut-bpy?ischemia–reperfusion [Rut-bpy?I/R]) with their mean arterial pressure (MAP) continuously monitored. The groups were submitted (SS?I/R and Rutbpy? I/R) or not (SS and Rut-bpy) to incomplete global brain ischemia by occlusion of the common bilateral carotid arteries during 30 min followed by reperfusion for further 60 min. Thirty minutes before ischemia, rats were treated pairwise by intraperitoneal injection of saline solution or Rut-bpy. At the end of experiments, brain was removed for triphenyltetrazolium chloride staining in order to quantify the total ischemic area. In a subset of rats, hippocampus was obtained for histopathology scoring, nitrate and nitrite measurements, immunostaining and western blotting of the nuclear factor- jB (NF-jB). Rutbpy pre-treatment decreased MAP variations during the transition from brain ischemia to reperfusion and decreased the fractional injury area. Rut-bpy pre-treatment reduced NF-jB hippocampal immunostaining and protein expression with improved histopathology scoring as compared to the untreated operated control. In conclusion, Rut-bpy improved the total brain infarction area and hippocampal neuronal viability in part by inhibiting NF-jB signaling and helped to stabilize the blood pressure during the transition from ischemia to reperfusion.Marcio Wilker Soares Campelo • Reinaldo Barreto Oria´ • Luiz Gonzaga de Franc¸a Lopes • Gerly Anne de Castro Brito • Armenio Aguiar dos Santos • Raquel Cavalcante de Vasconcelos • Francisco Ordelei Nascimento da Silva • Beatrice Nuto Nobrega • Moise´s Tolentino Bento-Silva • Paulo Roberto Leita˜o de VasconcelosMarcio Wilker Soares Campelo • Reinaldo Barreto Oria´ • Luiz Gonzaga de Franc¸a Lopes • Gerly Anne de Castro Brito • Armenio Aguiar dos Santos • Raquel Cavalcante de Vasconcelos • Francisco Ordelei Nascimento da Silva • Beatrice Nuto Nobrega • Moise´s Tolentino Bento-Silva • Paulo Roberto Leita˜o de Vasconcelos
URI: http://www.repositorio.ufc.br/handle/riufc/5698
ISSN: 0364-3190
Appears in Collections:DFIFA - Artigos publicados em revista científica

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