Please use this identifier to cite or link to this item:
Title in Portuguese: Anti-inflammatory and immunomodulatory effect of an extract of coccidioides posadasii in experimental arthritis
Author: Pinto, Ana Carolina Matias Dinelly
Cordeiro, Rossana de Aguiar
Sidrim, José Julio Costa
Leite, Ana Karine Rocha de Melo
Leite, Ana Caroline Rocha de Melo
Girão, Virgínia Cláudia Carneiro
Brilhante, Raimunda Sâmia Nogueira
Rocha, Marcos Fábio Gadelha
Cunha, Fernando de Queiroz
Rocha, Francisco Airton Castro
Keywords: Coccidioides
Óxido Nítrico
Issue Date: Apr-2013
Publisher: Mycopathologia
Citation: PINTO, A. C. M. D. et al. Anti-inflammatory and immunomodulatory effect of an extract of coccidioides posadasii in experimental arthritis. Mycopathologia, New York, v. 175, n. 3, p. 193–206, abr. 2013.
Abstract: Trying to surpass host defenses, fungal infections alter the immune response. Components from nonpathogenic fungi present therapeutic anti-inflammatory and immunomodulating activities. This study reveals that proteins present in a Coccidioides posadasii extract provide anti-inflammatory benefit in experimental arthritis. Zymosan was given intra-articularly to rats and mice, and groups were pretreated with C. posadasii extract either per os or intraperitoneally. Controls received the vehicle. Acute hypernociception was evaluated using articular incapacitation and von Frey methods. Cell influx and cytokine levels were assessed in joint exudates. Joint damage was evaluated by histopathology and determination of glycosaminoglycan content of the cartilage. Synovia was evaluated for cell death and inducible nitric oxide synthase (iNOS) expression using TUNEL and immunohistochemistry, respectively. Pretreatment with C. posadasii extract significantly inhibited acute and chronic cell influx, hypernociception, and provoked reduction of glycosaminoglycan loss while reducing chronic synovitis, cell death, and iNOS expression. Reduction/alkylation of C. posadasii extract abrogated these effects.C. posadasii administration did not alter TNF-a, IL-1b, IL-17, and c-interferon levels, whereas IL-10 levels were significantly reduced. Data reveal that a C. posadasii extract reduces iNOS expression that is associated with inhibition of synovial apoptosis and decrease in IL-10 levels released into zymosan-inflamed joints. Characterization of active components excluded charged carbohydrates while pointing to a protein as responsible for these effects. In summary, systemic administration of components from a pathogenic fungus provides anti-inflammatory effects, being species-independent and orally active. Besides adding to understand host response against fungi, the results may lead to therapeutic implications.
ISSN: 0301-486X Impressa
1573-0832 On line
Appears in Collections:DMC - Artigos publicados em revistas científicas

Files in This Item:
File Description SizeFormat 
2013_art_acmdpinto.pdf616,66 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.