Please use this identifier to cite or link to this item:
Title in Portuguese: p-Methoxycinnamic acid diesters lower dyslipidemia, liver oxidative stress and toxicity in high-fat diet fed mice and human peripheral blood lymphocytes
Author: Paim, Raquel Teixeira Terceiro
Rodrigues, Paula Salmito Alves
Silva, José Ytalo Gomes da
Paula Junior, Valdir Ferreira de
Silva, Bruno Bezerra da
Freitas, Claísa Andréa Silva de
Oriá, Reinaldo Barreto
Florean, Eridan Orlando Pereira Tramontina
Rondina, Davide
Guedes, Maria Izabel Florindo
Keywords: Hiperlipidemias
Estresse Oxidativo
Oxidative Stress
Issue Date: Jan-2020
Publisher: Nutrients
Citation: PAIM, R. T. T. p-Methoxycinnamic acid diesters lower dyslipidemia, liver oxidative stress and toxicity in high-fat diet fed mice and human peripheral blood lymphocytes. Nutrients, v. 12, n. 1, p. 1-20, jan. 2020.
Abstract: The pursuit of cholesterol lowering natural products with less side e ects is needed for controlling dyslipidemia and reducing the increasing toll of cardiovascular diseases that are associated with morbidity and mortality worldwide. The present study aimed at the examining e ects of p-methoxycinnamic acid diesters (PCO-C) from carnauba (Copernicia prunifera)-derived wax on cytotoxic, genotoxic responses in vitro and on dyslipidemia and liver oxidative stress in vivo, utilizing high-fat diet (HFD) chronically fed Swiss mice. In addition, we evaluated the e ect of PCO-C on the expression of key cholesterol metabolism-related genes, as well as the structural interactions between PCO-C and lecithin-cholesterol acyl transferase (LCAT) in silico. Oral treatment with PCO-C was able to reduce total serum cholesterol and low-density lipoprotein (LDL) levels following HFD. In addition, PCO-C reduced excessive weight gain and lipid peroxidation, and increased the gene expression of LCAT following HFD. Furthermore, the high a nity of the studied compound (DG: 􀀀8.78 Kcal/mol) towards the active sites of mutant LCAT owing to hydrophobic and van derWaals interactions was confirmed using bioinformatics. PCO-C showed no evidence of renal and hepatic toxicity, unlike simvastatin, that elevated aspartate aminotransferase (AST) levels, a marker of liver dysfunction. Finally, PCO-C showed no cytotoxicity or genotoxicity towards human peripheral blood lymphocytes in vitro. Our results suggest that PCO-C exerts hypocholesterolemic e ects. The safety of PCO-C in the toxicological tests performed and the reports of its beneficial biological e ects render this a promising compound for the development of new cholesterol-lowering therapeutics to control dyslipidemia. More work is needed for further elucidating PCO-C role on lipid metabolism to support future clinical studies.
metadata.dc.type: Artigo de Periódico
ISSN: 2072-6643
Appears in Collections:DMC - Artigos publicados em revistas científicas

Files in This Item:
File Description SizeFormat 
2020_art_rttpaim.pdf1,76 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.