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Title in Portuguese: Cefepime and amoxicillin increase metabolism and enhance caspofungin tolerance of Candida albicans biofilms
Author: Cordeiro, Rossana de Aguiar
Evangelista, Antonio Jose de Jesus
Serpa, Rosana
Andrade, Ana Raquel Colares de
Mendes, Patrícia Bruna Leite
Oliveira, Jonathas Sales de
Alencar, Lucas Pereira de
Pereira, Vandbergue Santos
Lima-Neto, Reginaldo Gonçalves
Brilhante, Raimunda Nogueira
Sidrim, José Júlio Costa
Maia, Débora Castelo Brancode Souza Collares
Rocha, Marcos Fábio Gadelha
Keywords: Antibacterianos
Anti-Bacterial Agents
Candida albicans
Issue Date: Jun-2019
Publisher: Frontiers in Microbiology
Citation: CORDEIRO, Rossana de Aguiar et al. Cefepime and amoxicillin increase metabolism and enhance caspofungin tolerance of Candida albicans biofilms. Front. Microbiol., v. 10, p. 1-9, jun. 2019.
Abstract: It is well known that prolonged antibiotic therapy alters the mucosal microbiota composition, increasing the risk of invasive fungal infection (IFI) in immunocompromised patients. The present study investigated the direct effect of b-lactam antibiotics cefepime (CEF) and amoxicillin (AMOX) on biofilm production by Candida albicans ATCC 10231. Antibacterials at the peak plasmatic concentration of each drug were tested against biofilms grown on polystyrene surfaces. Biofilms were evaluated for biomass production, metabolic activity, carbohydrate and protein contents, proteolytic activity, ultrastructure, and tolerance to antifungals. CEF and AMOX enhanced biofilm production by C. albicans ATCC 10231, stimulating biomass production, metabolic activity, viable cell counts, and proteolytic activity, as well as increased biovolume and thickness of these structures. Nevertheless, AMOX induced more significant changes in C. albicans biofilms than CEF. In addition, it was shown that AMOX increased the amount of chitin in these biofilms, making them more tolerant to caspofungin. Finally, it was seen that, in response to AMOX, C. albicans biofilms produce Hsp70 – a protein with chaperone function related to stressful conditions. These results may have a direct impact on the pathophysiology of opportunistic IFIs in patients at risk.
metadata.dc.type: Artigo de Periódico
ISSN: 1664-302X
Appears in Collections:DPML - Artigos publicados em revista científica

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