Parâmetros seminais e proteoma do sêmen de ariacó (Lutjanus synagris, Perciformes) tratados com gonadotropina coriônica humana (hcg)

Abstract

Human Chorionic Gonadotropine (hCG) enhances spawning in snappers, especially based on the production and quality of eggs and hatching success rates. The objective of the present study was to evaluate sperm parameters and semen proteome from lane snapper males subjected to hormonal therapy with hCG. Proteins were analyzed by SDS-PAGE prefractionation combined with indentification by ESI-Q-TOF mass spectrometry. Using this approach, we detected 30 band types with a total of 71 identified proteins in lane snapper semen. Creatine kinase, tubulin, heat shock cognate 71, heat shock protein 60, peroxiredoxin 1and 3 were present in high abundance in semen of lane snapper. PSP-I, HSP90AA1, ubiquitin carboxi-terminal hydrolase 5 and transferrin were differentially expressed in semen from hCH-treated fish. hCG increased sperm concentration, morphologically normal sperm, soluble protein concentration and five different bands in lane snapper semen. Most of lane snapper semen proteins present binding and catalytic activity (hydrolases and transferases). Semen proteins that were over expressed after hCG treatment are potentially involved in ATP storage, cellular composition and sperm protection. This is the first description of semen parameters and proteome of lane snapper semen after hCG treatment. Such discoveries open new opportunities for research to improve technologies for fish reproduction and preservation.