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Title in Portuguese: Validação de método por MEFS-HS/CG-EM para determinação de Carbaril em plasma sanguíneo
Title: Method Validation for HS-SPME/GC-MS for determination of Carbaryl in blood plasma
Author: Souza, Francisco Thiago C. de
Camelo, André Luiz M.
Marques, Ricardo de Araújo
F. Filho, Luiz Ivando Pires
Pinheiro, Ronald F.
Longhinotti, Elisane
Becker, Helena
Keywords: Carbaryl
Plasma (Blood Plasma)
Issue Date: Mar-2017
Publisher: Química Nova
Citation: SOUZA, F. T. C. et al. Validação de método por MEFS-HS/CG-EM para determinação de Carbaril em plasma sanguíneo. Química Nova, v. 40, n. 5, p. 586-593, 2017.
Abstract: METHOD VALIDATION FOR HS-SPME / GC-MS FOR DETERMINATION OF CARBARYL IN BLOOD PLASMA. The aim this research is to develop a HS-SPME/GC–MS method to analyze the carbaryl in blood plasma samples of the rural workers, who live round the perimeter of Chapada do Apodi-CE. This was achieved by chromatographic signal of the 1-naftol, degradation product of carbaryl with the intention of evaluating the degree of exposure of the workers to pesticides, causing chromosome abnormalities as already evidence in appropriate literature. Three different types of covered SPME fibers were tested and a full factorial design with central point in two levels. The variables optimized were temperature and ionic strength and temperature was the response in the design. Study was undertaken to determinate the equilibrium time. The thermal degradation of carbaryl in SPME headspace was analyzed by 1 H-NMR. Proposed method achieved low limits of detection and quantification (1.5 and 5 ng mL-1, respectively) with good precision and accuracy, using the method of weighted least squares. However, the proposed method can be considered a good alternative way of monitoring carbaryl in blood plasma by analytical signal of 1-naftol. None of the plasma analyzed presented a concentration of 1-naftol compound higher than limit of detection of the method proposed here.
ISSN: 0100-4042
Appears in Collections:DMC - Artigos publicados em revistas científicas

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