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Title in Portuguese: In vitro evaluation of anti-Leishmania and anti-Trypanosoma cruzi activity of viperidae venoms
Author: Rosas, Nathalia Santiago Cezar
Spencer, Patrick Jack
Carvalho, Camila Aparecida
Silveira, João Alison de Moraes
Vasconcelos-Filho, Francisco Sérgio Lopes
Monteiro, Helena Serra Azul
Bevilaqua, Claudia Maria Leal
Andrade Junior, Heitor Franco de
Evangelista, Janaina Serra Azul Monteiro
Keywords: Doença de Chagas
Chagas Disease
Trypanosoma cruzi
Issue Date: Aug-2016
Publisher: Asian Journal of Science and Technology
Citation: ROSAS, N. S. C. et al. In vitro evaluation of anti -leishmania and anti -trypanosoma cruzi activity of viperidae venoms. Asian Journal of Science and Technology, v. 7, n. 8, p.3362-3366, aug. 2016.
Abstract: The chemotherapy available for the treatment of Leishmaniosis and Chagas is restricted to a few medicines. Moreover, snake venoms have been studied because they have active substances with therapeutic potential. Thus, the aim of this study was to evaluate the in vitro effects of total venom of Crotalusdurissuscascavella , Crotalusdurissusterrificus and Bothropsjararaca in Leishmania chagasi and Trypanosoma cruzi .RAW264.7 and LLC - MK2 cells, L. chagasi promastigote and trypomastigotes of T. cruzi. In L.chagasi amastigotes of T. cruzi was carried out in situ ELISA test and MTT test. The toxins concentration of 500 μg/mL showed 100% mortality on the cells studied. Since of the 250 μg/mL concentration had obtained less than 50% cytotoxicity. These results presente d high toxicity in trypomastigotes and amastigotes of T.cruzi . In promastigotes, the venom of C. d. terrificus caused 71.1% mortality at a concentration of 250 μg/mL and the IC50 of 63.5 to 155.0 mg/mL. In L. chagasi amastigotes, 250 μg/mL the Bothrops jar araca venom caused 96% mortality and a lower IC50 value with the range of 55.6 to 90.9 μg/mL. Therefore, the venoms of C. d. terrificus and B. jararaca in the concentrations tested had anti - Leishmaniosis and anti - Chagas disease activity
metadata.dc.type: Artigo de Periódico
ISSN: 0976 - 3376
Appears in Collections:PPGF - Artigos publicados em revistas científica

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