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http://repositorio.ufc.br/handle/riufc/17731
Tipo: | Artigo de Periódico |
Título: | Deregulated expression of SRC, LYN and CKB kinases by DNA methylation and its potential role in gastric cancer Invasiveness and metastasis |
Autor(es): | Mello, Adriano Azevedo Leal, Mariana Ferreira Rey, Juan Antonio Pinto, Giovanny Rebouças Lamarão, Leticia Martins Montenegro, Raquel Carvalho Alves, Ana Paula Negreiros Nunes Assumpção, Paulo Pimentel Borges, Barbara do Nascimento Smith, Marília Cardoso Burbano, Rommel Rodriguez |
Palavras-chave: | Neoplasias Gástricas;DNA |
Data do documento: | Out-2015 |
Instituição/Editor/Publicador: | Plos One |
Citação: | MELLO, A. A. et al. Deregulated expression of SRC, LYN and CKB kinases by DNA methylation and Its potential role in gastric cancer invasiveness and metastasis. Plos One, San Francisco, v. 10, p. 1-23, out. 2015. |
Abstract: | Kinases are downstream modulators and effectors of several cellular signaling cascades and play key roles in the development of neoplastic disease. In this study, we aimed to evaluate SRC, LYN and CKB protein and mRNA expression, as well as their promoter methylation, in gastric cancer. We found elevated expression of SRC and LYN kinase mRNA and protein but decreased levels of CKB kinase, alterations that may have a role in the invasiveness and metastasis of gastric tumors. Expression of the three studied kinases was also associated with MYC oncogene expression, a possible biomarker for gastric cancer. To understand the mechanisms that regulate the expression of these genes, we evaluated the DNA promoter methylation of the three kinases. We found that reduced SRC and LYN methylation and increased CKB methylation was associated with gastric cancer. The reduced SRC and LYN methylation was associated with increased levels of mRNA and protein expression, suggesting that DNA methylation is involved in regulating the expression of these kinases. Conversely, reduced CKB methylation was observed in samples with reduced mRNA and protein expression, suggesting CKB expression was found to be only partly regulated by DNA methylation. Additionally, we found that alterations in the DNA methylation pattern of the three studied kinases were also associated with the gastric cancer onset, advanced gastric cancer, deeper tumor invasion and the presence of metastasis. Therefore, SRC, LYN and CKB expression or DNA methylation could be useful markers for predicting tumor progression and targeting in anti-cancer strategies. |
URI: | http://www.repositorio.ufc.br/handle/riufc/17731 |
ISSN: | 1932-6203 |
Aparece nas coleções: | PPGF - Artigos publicados em revistas científica |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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2015_art_aamello.pdf | 2,43 MB | Adobe PDF | Visualizar/Abrir |
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