Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/10328
Title in Portuguese: (−)-α-Bisabolol inhibits preferentially electromechanical coupling on rat isolated arteries
Author: Siqueira, Rodrigo José Bezerra de
Ribeiro-Filho, Helder Veras
Freire, R. S.
Freire, W. B. S.
Vasconcelos-Silva, A. A.
Soares, M. A.
Lahlou, Saad
Magalhães, Pedro Jorge Caldas
Cosker, François
Keywords: Músculo Liso Vascular
Sistema Cardiovascular
Issue Date: Oct-2014
Publisher: Vascular Pharmacology
Citation: SIQUEIRA, R, J. B. de et al. (−)-α-Bisabolol inhibits preferentially electromechanical coupling on rat isolated arteries. Vascular Pharmacology, v. 63, n. 1, p. 37-45, out. 2014.
Abstract: Previous findings enable us to hypothesize that (−)-α-bisabolol acts as inhibitor of voltage-dependent Ca2+ channels in smooth muscle. The current studywas aimed at consolidating such hypothesis through the recording of isometric tension, measurement of intracellular Ca2+ as well as discovery of channel target using in silico analysis. In rat aortic rings, (−)-α-bisabolol (1–1000 μM) relaxed KCl- and phenylephrine-elicited contractions, but the IC50 differed significantly (22.8 [17.6–27.7] and 200.7 [120.4–334.6] μM, respectively). The relaxation of phenylephrine contractions remained unaffected by L-NAME, indomethacin, 1H-[1,2,4]oxadiazolo[4,3-a] quinoxalin-1-one, tetraethylammonium, glibenclamide or KT-5720. Under Ca2+-free conditions, (−)-α- bisabolol did not alter the contractions evoked by phenylephrine or caffeine whereas it reduced those evoked by CaCl2 in KCl-, but not in PHE-stimulated preparations. Furthermore, it did not significantly alter the contractions evoked by phorbol 12,13-dibutyrate or induced by the extracellular Ca2+ restoration in cyclopiazonic acidtreated preparations. In mesenteric rings loaded with Fluo-4 AM, (−)-α-bisabolol blunted the tension and the cytosolic levels of Ca2+ in response to K+ but not to norepinephrine. Silico docking analysis of the Cavβ2a subunit of voltage-dependent Ca2+ channel indicated putative docking sites for (−)-α-bisabolol. These findings reinforce the ability of (−)-α-bisabolol to inhibit preferentially contractile responses evoked by Ca2+ influx through voltage-dependent Ca2+ channels.
URI: http://www.repositorio.ufc.br/handle/riufc/10328
ISSN: 1537-1891
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