Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/10190
Title in Portuguese: Antimicrobial effect of Dinoponera quadriceps (Hymenoptera: Formicidae) venom against Staphylococcus aureus strains
Author: Lima, Danya Bandeira
Torres, Alba Fabíola Costa
Mello, Clarissa Perdigão
Menezes, Ramon Róseo Paula Pessoa Bezerra
Sampaio, Tiago Lima
Canuto, Jader Almeida
Silva, José Júnior Alves da
Freire, Valder Nogueira
Quinet, Yves Patric
Havt, Alexandre
Monteiro, Helena Serra Azul
Nogueira, Nadia Accioly Pinto
Martins, Alice Maria Costa
Keywords: Staphylococcus
Venenos
Issue Date: Aug-2014
Publisher: Journal of Applied Microbiology
Citation: LIMA, D. B. et al. Antimicrobial effect of Dinoponera quadriceps (Hymenoptera : Formicidae) venom against Staphylococcus aureus strains. Journal of Applied Microbiology, Oxford, v. 117, n. 2, p. 390-396, ago. 2014.
Abstract: Aims: Dinoponera quadriceps venom (DqV) was examined to evaluate the antibacterial activity and its bactericidal action mechanism against Staphylococcus aureus. Methods and Results: DqV was tested against a standard strain of methicillinsensitive Staphylococcus aureus (MSSA), Staph. aureus ATCC 6538P and two standard strains of methicillin-resistant Staphylococcus aureus (MRSA), Staph. aureus ATCC 33591 and Staph. aureus CCBH 5330. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the rate of kill and pH sensitivity of the DqV were determined by microdilution tests. Bactericidal and inhibitory concentrations of DqV were tested to check its action on Staph. aureus membrane permeability and cell morphology. The MIC and MBC of DqV were 6 25 and 12 5 lg ml 1 for Staph. aureus ATCC 6538P, 12 5 and 50 lg ml 1 for Staph. aureus CCBH 5330 and 100 and 100 lg ml 1 for Staph. aureus ATCC 33591, respectively. Complete bacterial growth inhibition was observed after 4 h of incubation with the MBC of DqV. A lowest MIC was observed in alkaline pH. Alteration in membrane permeability was observed through the increase in crystal violet uptake, genetic material release and morphology in atomic force microscopy. Conclusions: The results suggest antibacterial activity of DqV against Staph. aureus and that the venom acts in the cell membrane. Significance and Impact of the Study: Alteration in membrane permeability may be associated with the antimicrobial activity of hymenopteran venoms.
URI: http://www.repositorio.ufc.br/handle/riufc/10190
ISSN: 1365-2672 Online
1364-5072 Impresso
Appears in Collections:DFIFA - Artigos publicados em revista científica

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